Synthesis of a trimeric gp120 epitope mimic conjugated to a T-helper peptide to improve antigenicity

Joan G. Schellinger, Lieza M. Danan-Leon, Jessica A. Hoch, Aemro Kassa, Indresh Srivastava, David Davis, Jacquelyn Gervay-Hague

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A fully synthetic trivalent mimotope of gp120 conjugated to pan allelic HLA DR binding epitope was prepared using solid-phase peptide synthesis and optimized copper-catalyzed azide-alkyne cycloaddition. The methodology efficiently provides chemically uniform heteromultimeric peptide constructs with enhanced binding, avidity, and specificity toward an established HIV-neutralizing human antibody, MAb b12. The versatile synthetic strategy serves as a powerful platform for the development of synthetic peptides as potential HIV-1 vaccine candidates.

Original languageEnglish (US)
Pages (from-to)3230-3233
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Issue number10
DOIs
StatePublished - Mar 16 2011

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Peptide T
Epitopes
Peptides
AIDS Vaccines
Solid-Phase Synthesis Techniques
Alkynes
Azides
Cycloaddition Reaction
Neutralizing Antibodies
HIV-1
Copper
Vaccines
Cycloaddition
HIV
Antibodies
PADRE 45

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Synthesis of a trimeric gp120 epitope mimic conjugated to a T-helper peptide to improve antigenicity. / Schellinger, Joan G.; Danan-Leon, Lieza M.; Hoch, Jessica A.; Kassa, Aemro; Srivastava, Indresh; Davis, David; Gervay-Hague, Jacquelyn.

In: Journal of the American Chemical Society, Vol. 133, No. 10, 16.03.2011, p. 3230-3233.

Research output: Contribution to journalArticle

Schellinger, Joan G. ; Danan-Leon, Lieza M. ; Hoch, Jessica A. ; Kassa, Aemro ; Srivastava, Indresh ; Davis, David ; Gervay-Hague, Jacquelyn. / Synthesis of a trimeric gp120 epitope mimic conjugated to a T-helper peptide to improve antigenicity. In: Journal of the American Chemical Society. 2011 ; Vol. 133, No. 10. pp. 3230-3233.
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