Abstract
α-Gal epitopes are carbohydrate structures bearing an α-D-Galp-(1 → 3)-β-D-Galp terminus and are the main cause of antibody-mediated hyperacute rejection in xenotransplantation. Nine monosaccharides and ten disaccharides were evaluated as substrates for a fusion protein, which contains both α-(1 → 3)-galactosyltransferase and uridine-5′-diphosphogalactose 4-epimerase. Four disaccharide and six trisaccharide α-Gal epitope derivatives were synthesized utilizing this novel fusion enzyme.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 873-878 |
| Number of pages | 6 |
| Journal | Carbohydrate Research |
| Volume | 329 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 1 2000 |
| Externally published | Yes |
Keywords
- Enzymatic glycosylation
- Epitopes
- Fusion protein
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Organic Chemistry
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Cite this
Fang, J., Chen, X., Zhang, W., Janczuk, A., & Wang, P. G. (2000). Synthesis of α-Gal epitope derivatives with a galactosyltransferase-epimerase fusion enzyme. Carbohydrate Research, 329(4), 873-878. https://doi.org/10.1016/S0008-6215(00)00245-7