Synthesis of α-Gal epitope derivatives with a galactosyltransferase-epimerase fusion enzyme

Jianwen Fang, Xi Chen, Wei Zhang, Adam Janczuk, Peng George Wang

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

α-Gal epitopes are carbohydrate structures bearing an α-D-Galp-(1 → 3)-β-D-Galp terminus and are the main cause of antibody-mediated hyperacute rejection in xenotransplantation. Nine monosaccharides and ten disaccharides were evaluated as substrates for a fusion protein, which contains both α-(1 → 3)-galactosyltransferase and uridine-5′-diphosphogalactose 4-epimerase. Four disaccharide and six trisaccharide α-Gal epitope derivatives were synthesized utilizing this novel fusion enzyme.

Original languageEnglish (US)
Pages (from-to)873-878
Number of pages6
JournalCarbohydrate Research
Volume329
Issue number4
DOIs
StatePublished - Dec 1 2000
Externally publishedYes

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Keywords

  • Enzymatic glycosylation
  • Epitopes
  • Fusion protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry

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