Synthesis of α-Gal epitope derivatives with a galactosyltransferase-epimerase fusion enzyme

Jianwen Fang; Xi Chen; Wei Zhang; Adam Janczuk; Peng George Wang

doi:10.1016/S0008-6215(00)00245-7

Synthesis of α-Gal epitope derivatives with a galactosyltransferase-epimerase fusion enzyme

Jianwen Fang, Xi Chen, Wei Zhang, Adam Janczuk, Peng George Wang

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

α-Gal epitopes are carbohydrate structures bearing an α-D-Galp-(1 → 3)-β-D-Galp terminus and are the main cause of antibody-mediated hyperacute rejection in xenotransplantation. Nine monosaccharides and ten disaccharides were evaluated as substrates for a fusion protein, which contains both α-(1 → 3)-galactosyltransferase and uridine-5′-diphosphogalactose 4-epimerase. Four disaccharide and six trisaccharide α-Gal epitope derivatives were synthesized utilizing this novel fusion enzyme.

Original language	English (US)
Pages (from-to)	873-878
Number of pages	6
Journal	Carbohydrate Research
Volume	329
Issue number	4
DOIs	https://doi.org/10.1016/S0008-6215(00)00245-7
State	Published - Dec 1 2000
Externally published	Yes

Keywords

Enzymatic glycosylation
Epitopes
Fusion protein

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Organic Chemistry

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title = "Synthesis of α-Gal epitope derivatives with a galactosyltransferase-epimerase fusion enzyme",

abstract = "α-Gal epitopes are carbohydrate structures bearing an α-D-Galp-(1 → 3)-β-D-Galp terminus and are the main cause of antibody-mediated hyperacute rejection in xenotransplantation. Nine monosaccharides and ten disaccharides were evaluated as substrates for a fusion protein, which contains both α-(1 → 3)-galactosyltransferase and uridine-5′-diphosphogalactose 4-epimerase. Four disaccharide and six trisaccharide α-Gal epitope derivatives were synthesized utilizing this novel fusion enzyme.",

keywords = "Enzymatic glycosylation, Epitopes, Fusion protein",

author = "Jianwen Fang and Xi Chen and Wei Zhang and Adam Janczuk and Wang, {Peng George}",

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T1 - Synthesis of α-Gal epitope derivatives with a galactosyltransferase-epimerase fusion enzyme

AU - Fang, Jianwen

AU - Chen, Xi

AU - Zhang, Wei

AU - Janczuk, Adam

AU - Wang, Peng George

PY - 2000/12/1

Y1 - 2000/12/1

N2 - α-Gal epitopes are carbohydrate structures bearing an α-D-Galp-(1 → 3)-β-D-Galp terminus and are the main cause of antibody-mediated hyperacute rejection in xenotransplantation. Nine monosaccharides and ten disaccharides were evaluated as substrates for a fusion protein, which contains both α-(1 → 3)-galactosyltransferase and uridine-5′-diphosphogalactose 4-epimerase. Four disaccharide and six trisaccharide α-Gal epitope derivatives were synthesized utilizing this novel fusion enzyme.

AB - α-Gal epitopes are carbohydrate structures bearing an α-D-Galp-(1 → 3)-β-D-Galp terminus and are the main cause of antibody-mediated hyperacute rejection in xenotransplantation. Nine monosaccharides and ten disaccharides were evaluated as substrates for a fusion protein, which contains both α-(1 → 3)-galactosyltransferase and uridine-5′-diphosphogalactose 4-epimerase. Four disaccharide and six trisaccharide α-Gal epitope derivatives were synthesized utilizing this novel fusion enzyme.

KW - Enzymatic glycosylation

KW - Epitopes

KW - Fusion protein

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