Abstract
Rice seed callus expressed and secreted α-amylase at high levels. Twenty percent of the protein secreted by the callus was α-amylase. The callus secreted about 840 μg α-amylase with 10.9 x 103 units of activity per gram dry weight callus per day. The α-amylase from callus exhibited a more complex isoform pattern than the germinating seed α-amylase. In addition, the level of mRNA expression by the five α-amylase gene groups was markedly different between callus and the germinating seed. The rice callus culture has features which make it attractive as a potential system for expressing proteins in plant cell fermentation systems.
Original language | English (US) |
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Pages (from-to) | 545-551 |
Number of pages | 7 |
Journal | Biotechnology and Bioengineering |
Volume | 38 |
Issue number | 5 |
State | Published - 1991 |
ASJC Scopus subject areas
- Biotechnology
- Microbiology