Synthesis and secretion of α-amylase by rice callus: Evidence for differential gene expression

C. R. Simmons, N. Huang, Y. Cao, R. L. Rodriguez

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Rice seed callus expressed and secreted α-amylase at high levels. Twenty percent of the protein secreted by the callus was α-amylase. The callus secreted about 840 μg α-amylase with 10.9 x 103 units of activity per gram dry weight callus per day. The α-amylase from callus exhibited a more complex isoform pattern than the germinating seed α-amylase. In addition, the level of mRNA expression by the five α-amylase gene groups was markedly different between callus and the germinating seed. The rice callus culture has features which make it attractive as a potential system for expressing proteins in plant cell fermentation systems.

Original languageEnglish (US)
Pages (from-to)545-551
Number of pages7
JournalBiotechnology and Bioengineering
Volume38
Issue number5
StatePublished - 1991

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

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