Synthesis and secretion of α-amylase by rice callus: Evidence for differential gene expression

C. R. Simmons, N. Huang, Y. Cao, R. L. Rodriguez

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Rice seed callus expressed and secreted α-amylase at high levels. Twenty percent of the protein secreted by the callus was α-amylase. The callus secreted about 840 μg α-amylase with 10.9 x 103 units of activity per gram dry weight callus per day. The α-amylase from callus exhibited a more complex isoform pattern than the germinating seed α-amylase. In addition, the level of mRNA expression by the five α-amylase gene groups was markedly different between callus and the germinating seed. The rice callus culture has features which make it attractive as a potential system for expressing proteins in plant cell fermentation systems.

Original languageEnglish (US)
Pages (from-to)545-551
Number of pages7
JournalBiotechnology and Bioengineering
Volume38
Issue number5
StatePublished - 1991

Fingerprint

Amylases
Bony Callus
Gene expression
Gene Expression
Seed
Seeds
Proteins
Plant Cells
Fermentation
Oryza
Protein Isoforms
Genes
Messenger RNA
Weights and Measures

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Synthesis and secretion of α-amylase by rice callus : Evidence for differential gene expression. / Simmons, C. R.; Huang, N.; Cao, Y.; Rodriguez, R. L.

In: Biotechnology and Bioengineering, Vol. 38, No. 5, 1991, p. 545-551.

Research output: Contribution to journalArticle

Simmons, CR, Huang, N, Cao, Y & Rodriguez, RL 1991, 'Synthesis and secretion of α-amylase by rice callus: Evidence for differential gene expression', Biotechnology and Bioengineering, vol. 38, no. 5, pp. 545-551.
Simmons, C. R. ; Huang, N. ; Cao, Y. ; Rodriguez, R. L. / Synthesis and secretion of α-amylase by rice callus : Evidence for differential gene expression. In: Biotechnology and Bioengineering. 1991 ; Vol. 38, No. 5. pp. 545-551.
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