Abstract
Rice seed callus expressed and secreted α-amylase at high levels. Twenty percent of the protein secreted by the callus was α-amylase. The callus secreted about 840 μg α-amylase with 10.9 x 103 units of activity per gram dry weight callus per day. The α-amylase from callus exhibited a more complex isoform pattern than the germinating seed α-amylase. In addition, the level of mRNA expression by the five α-amylase gene groups was markedly different between callus and the germinating seed. The rice callus culture has features which make it attractive as a potential system for expressing proteins in plant cell fermentation systems.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 545-551 |
| Number of pages | 7 |
| Journal | Biotechnology and Bioengineering |
| Volume | 38 |
| Issue number | 5 |
| State | Published - 1991 |
ASJC Scopus subject areas
- Biotechnology
- Microbiology
Fingerprint Dive into the research topics of 'Synthesis and secretion of α-amylase by rice callus: Evidence for differential gene expression'. Together they form a unique fingerprint.
Cite this
Simmons, C. R., Huang, N., Cao, Y., & Rodriguez, R. L. (1991). Synthesis and secretion of α-amylase by rice callus: Evidence for differential gene expression. Biotechnology and Bioengineering, 38(5), 545-551.