Abstract
Rice seed callus expressed and secreted α-amylase at high levels. Twenty percent of the protein secreted by the callus was α-amylase. The callus secreted about 840 μg α-amylase with 10.9 x 103 units of activity per gram dry weight callus per day. The α-amylase from callus exhibited a more complex isoform pattern than the germinating seed α-amylase. In addition, the level of mRNA expression by the five α-amylase gene groups was markedly different between callus and the germinating seed. The rice callus culture has features which make it attractive as a potential system for expressing proteins in plant cell fermentation systems.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 545-551 |
| Number of pages | 7 |
| Journal | Biotechnology and Bioengineering |
| Volume | 38 |
| Issue number | 5 |
| State | Published - 1991 |
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ASJC Scopus subject areas
- Biotechnology
- Microbiology
Cite this
Synthesis and secretion of α-amylase by rice callus : Evidence for differential gene expression. / Simmons, C. R.; Huang, N.; Cao, Y.; Rodriguez, R. L.
In: Biotechnology and Bioengineering, Vol. 38, No. 5, 1991, p. 545-551.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synthesis and secretion of α-amylase by rice callus
T2 - Evidence for differential gene expression
AU - Simmons, C. R.
AU - Huang, N.
AU - Cao, Y.
AU - Rodriguez, R. L.
PY - 1991
Y1 - 1991
N2 - Rice seed callus expressed and secreted α-amylase at high levels. Twenty percent of the protein secreted by the callus was α-amylase. The callus secreted about 840 μg α-amylase with 10.9 x 103 units of activity per gram dry weight callus per day. The α-amylase from callus exhibited a more complex isoform pattern than the germinating seed α-amylase. In addition, the level of mRNA expression by the five α-amylase gene groups was markedly different between callus and the germinating seed. The rice callus culture has features which make it attractive as a potential system for expressing proteins in plant cell fermentation systems.
AB - Rice seed callus expressed and secreted α-amylase at high levels. Twenty percent of the protein secreted by the callus was α-amylase. The callus secreted about 840 μg α-amylase with 10.9 x 103 units of activity per gram dry weight callus per day. The α-amylase from callus exhibited a more complex isoform pattern than the germinating seed α-amylase. In addition, the level of mRNA expression by the five α-amylase gene groups was markedly different between callus and the germinating seed. The rice callus culture has features which make it attractive as a potential system for expressing proteins in plant cell fermentation systems.
UR - http://www.scopus.com/inward/record.url?scp=0026206709&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026206709&partnerID=8YFLogxK
M3 - Article
C2 - 18604813
AN - SCOPUS:0026206709
VL - 38
SP - 545
EP - 551
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 5
ER -