Synthesis and screening of a 'one-bead-one-compound' combinatorial peptide library

Kit Lam, M. Lebl

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

By using a 'split-synthesis' method in conjunction with standard solid- phase peptide synthesis, a library of peptide beads can be synthesized so there is only one unique peptide species on each bead-the 'one-bead-one- compound' concept. With an appropriate screening method targeted for a specific biological, physical, or biochemical property, a unique peptide bead can be identified, physically isolated, and the peptide structure determined. The screening method may involve (1) on-bead assay such as direct binding or covalent modification or (2) releasable assay in which the peptides are released from the bead for biological or biochemical testing. Examples of these methods for various targets will be described. This one-bead-one- peptide library method enables one to synthesize and screen 107 unique peptides routinely and rapidly and has proved to be an invaluable tool for basic research and drug discovery.

Original languageEnglish (US)
Pages (from-to)46-56
Number of pages11
JournalMethods in Molecular and Cellular Biology
Volume6
Issue number1
StatePublished - 1996
Externally publishedYes

Fingerprint

Peptide Library
Screening
Peptides
Assays
Solid-Phase Synthesis Techniques
Drug Discovery
Research
Testing

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Molecular Biology

Cite this

Synthesis and screening of a 'one-bead-one-compound' combinatorial peptide library. / Lam, Kit; Lebl, M.

In: Methods in Molecular and Cellular Biology, Vol. 6, No. 1, 1996, p. 46-56.

Research output: Contribution to journalArticle

@article{5000ae4f2c584c5789a8bb1bfe952228,
title = "Synthesis and screening of a 'one-bead-one-compound' combinatorial peptide library",
abstract = "By using a 'split-synthesis' method in conjunction with standard solid- phase peptide synthesis, a library of peptide beads can be synthesized so there is only one unique peptide species on each bead-the 'one-bead-one- compound' concept. With an appropriate screening method targeted for a specific biological, physical, or biochemical property, a unique peptide bead can be identified, physically isolated, and the peptide structure determined. The screening method may involve (1) on-bead assay such as direct binding or covalent modification or (2) releasable assay in which the peptides are released from the bead for biological or biochemical testing. Examples of these methods for various targets will be described. This one-bead-one- peptide library method enables one to synthesize and screen 107 unique peptides routinely and rapidly and has proved to be an invaluable tool for basic research and drug discovery.",
author = "Kit Lam and M. Lebl",
year = "1996",
language = "English (US)",
volume = "6",
pages = "46--56",
journal = "Methods in Molecular and Cellular Biology",
issn = "0898-7750",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - Synthesis and screening of a 'one-bead-one-compound' combinatorial peptide library

AU - Lam, Kit

AU - Lebl, M.

PY - 1996

Y1 - 1996

N2 - By using a 'split-synthesis' method in conjunction with standard solid- phase peptide synthesis, a library of peptide beads can be synthesized so there is only one unique peptide species on each bead-the 'one-bead-one- compound' concept. With an appropriate screening method targeted for a specific biological, physical, or biochemical property, a unique peptide bead can be identified, physically isolated, and the peptide structure determined. The screening method may involve (1) on-bead assay such as direct binding or covalent modification or (2) releasable assay in which the peptides are released from the bead for biological or biochemical testing. Examples of these methods for various targets will be described. This one-bead-one- peptide library method enables one to synthesize and screen 107 unique peptides routinely and rapidly and has proved to be an invaluable tool for basic research and drug discovery.

AB - By using a 'split-synthesis' method in conjunction with standard solid- phase peptide synthesis, a library of peptide beads can be synthesized so there is only one unique peptide species on each bead-the 'one-bead-one- compound' concept. With an appropriate screening method targeted for a specific biological, physical, or biochemical property, a unique peptide bead can be identified, physically isolated, and the peptide structure determined. The screening method may involve (1) on-bead assay such as direct binding or covalent modification or (2) releasable assay in which the peptides are released from the bead for biological or biochemical testing. Examples of these methods for various targets will be described. This one-bead-one- peptide library method enables one to synthesize and screen 107 unique peptides routinely and rapidly and has proved to be an invaluable tool for basic research and drug discovery.

UR - http://www.scopus.com/inward/record.url?scp=0029908992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029908992&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 46

EP - 56

JO - Methods in Molecular and Cellular Biology

JF - Methods in Molecular and Cellular Biology

SN - 0898-7750

IS - 1

ER -