Synthesis and myocardial kinetics of N-13 and C-11 labeled branched-chain l-amino acids

J. R. Barrio, F. J. Baumgartner, E. Henze, M. S. Stauber, J. E. Egbert, N. S. MacDonald, H. R. Schelbert, M. E. Phelps, Fu-Tong Liu

Research output: Contribution to journalArticle

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Abstract

Glutamate dehydrogenase (GDH), immobilized on CNBr-activated Sepharose supports, was used with N-13 ammonia to aminate α-ketoisocaproic acid (KIC), and α-ketoisovaleric acid (KIV) to produce N-13-labeled branched-chain L-amino acids with radiochemical yields ranging from 29% to 35%. From kinetic and practical considerations, pH 7.5-8.0 was established to be optimal for the synthesis of N-13-labeled branched-chain-L-amino acids. Myocardial time-activity curves in dogs at control, during low-flow ischemia, reperfusion, and after transaminase inhibition following intracoronary bolus injection of the N-13-labeled amino acids were biexponential. Higher retention of N-13 activity was observed in ischemic segments both during low-flow ischemia (29.2%) and reperfusion (23.2%) when compared with controls (20.0%), (n = 4). On the other hand, transaminase inhibition decreased residue fractions from 21.0% at control to 13.9% (n = 4). The residual activity with L-[1-11C]leucine allows for the calculation of protein synthesis rates.

Original languageEnglish (US)
Pages (from-to)937-944
Number of pages8
JournalJournal of Nuclear Medicine
Volume24
Issue number10
StatePublished - 1983
Externally publishedYes

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Branched Chain Amino Acids
Transaminases
Reperfusion
Ischemia
Glutamate Dehydrogenase
Acids
Ammonia
Leucine
Sepharose
Dogs
Amino Acids
Injections
Proteins

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Barrio, J. R., Baumgartner, F. J., Henze, E., Stauber, M. S., Egbert, J. E., MacDonald, N. S., ... Liu, F-T. (1983). Synthesis and myocardial kinetics of N-13 and C-11 labeled branched-chain l-amino acids. Journal of Nuclear Medicine, 24(10), 937-944.

Synthesis and myocardial kinetics of N-13 and C-11 labeled branched-chain l-amino acids. / Barrio, J. R.; Baumgartner, F. J.; Henze, E.; Stauber, M. S.; Egbert, J. E.; MacDonald, N. S.; Schelbert, H. R.; Phelps, M. E.; Liu, Fu-Tong.

In: Journal of Nuclear Medicine, Vol. 24, No. 10, 1983, p. 937-944.

Research output: Contribution to journalArticle

Barrio, JR, Baumgartner, FJ, Henze, E, Stauber, MS, Egbert, JE, MacDonald, NS, Schelbert, HR, Phelps, ME & Liu, F-T 1983, 'Synthesis and myocardial kinetics of N-13 and C-11 labeled branched-chain l-amino acids', Journal of Nuclear Medicine, vol. 24, no. 10, pp. 937-944.
Barrio JR, Baumgartner FJ, Henze E, Stauber MS, Egbert JE, MacDonald NS et al. Synthesis and myocardial kinetics of N-13 and C-11 labeled branched-chain l-amino acids. Journal of Nuclear Medicine. 1983;24(10):937-944.
Barrio, J. R. ; Baumgartner, F. J. ; Henze, E. ; Stauber, M. S. ; Egbert, J. E. ; MacDonald, N. S. ; Schelbert, H. R. ; Phelps, M. E. ; Liu, Fu-Tong. / Synthesis and myocardial kinetics of N-13 and C-11 labeled branched-chain l-amino acids. In: Journal of Nuclear Medicine. 1983 ; Vol. 24, No. 10. pp. 937-944.
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