Abstract
In this paper, we describe the synthesis and characterization of 2,5-dimercaptobenzoic acid as a novel pH-sensitive disulfide reporter molecule for surface-enhanced Raman scattering (SERS) capable of inducing the controlled aggregation of gold (Au) colloids in solution without the addition of salts. While weak acids have been shown to yield some pH sensitivity as reporter molecules for SERS measurements, the reproducibility and signal strength of nanoparticle probes based on such molecules can vary greatly. This limited reproducibility depends greatly on the salt-induced aggregation of the colloidal nanoprobes, which is required in order to obtain SERS signals strong enough to probe individual clusters. This complicates their use in live cell sensing applications. We show that our approach results in primarily bridged nanoparticles comprising a pH-sensitive nanoprobe that can quantify accurately pH values well below 5.5. The robustness and sensitivity of this system makes it a powerful tool for measuring pH values on the nanoscale under in vitro conditions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3574-3580 |
| Number of pages | 7 |
| Journal | Analytical Chemistry |
| Volume | 84 |
| Issue number | 8 |
| DOIs | |
| State | Published - Apr 17 2012 |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Synthesis and characterization of a disulfide reporter molecule for enhancing pH measurements based on surface-enhanced raman scattering. / Lawson, Latevi; Huser, Thomas R.
In: Analytical Chemistry, Vol. 84, No. 8, 17.04.2012, p. 3574-3580.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synthesis and characterization of a disulfide reporter molecule for enhancing pH measurements based on surface-enhanced raman scattering
AU - Lawson, Latevi
AU - Huser, Thomas R
PY - 2012/4/17
Y1 - 2012/4/17
N2 - In this paper, we describe the synthesis and characterization of 2,5-dimercaptobenzoic acid as a novel pH-sensitive disulfide reporter molecule for surface-enhanced Raman scattering (SERS) capable of inducing the controlled aggregation of gold (Au) colloids in solution without the addition of salts. While weak acids have been shown to yield some pH sensitivity as reporter molecules for SERS measurements, the reproducibility and signal strength of nanoparticle probes based on such molecules can vary greatly. This limited reproducibility depends greatly on the salt-induced aggregation of the colloidal nanoprobes, which is required in order to obtain SERS signals strong enough to probe individual clusters. This complicates their use in live cell sensing applications. We show that our approach results in primarily bridged nanoparticles comprising a pH-sensitive nanoprobe that can quantify accurately pH values well below 5.5. The robustness and sensitivity of this system makes it a powerful tool for measuring pH values on the nanoscale under in vitro conditions.
AB - In this paper, we describe the synthesis and characterization of 2,5-dimercaptobenzoic acid as a novel pH-sensitive disulfide reporter molecule for surface-enhanced Raman scattering (SERS) capable of inducing the controlled aggregation of gold (Au) colloids in solution without the addition of salts. While weak acids have been shown to yield some pH sensitivity as reporter molecules for SERS measurements, the reproducibility and signal strength of nanoparticle probes based on such molecules can vary greatly. This limited reproducibility depends greatly on the salt-induced aggregation of the colloidal nanoprobes, which is required in order to obtain SERS signals strong enough to probe individual clusters. This complicates their use in live cell sensing applications. We show that our approach results in primarily bridged nanoparticles comprising a pH-sensitive nanoprobe that can quantify accurately pH values well below 5.5. The robustness and sensitivity of this system makes it a powerful tool for measuring pH values on the nanoscale under in vitro conditions.
UR - http://www.scopus.com/inward/record.url?scp=84859894071&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84859894071&partnerID=8YFLogxK
U2 - 10.1021/ac203103s
DO - 10.1021/ac203103s
M3 - Article
C2 - 22455337
AN - SCOPUS:84859894071
VL - 84
SP - 3574
EP - 3580
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 8
ER -