Synthesis and biological evaluation of geminal disulfones as HIV-1 integrase inhibitors

D. Christopher Meadows, Timothy B. Mathews, Thomas W. North, Michael J. Hadd, Chih Lin Kuo, Nouri Neamati, Jacquelyn Gervay-Hague

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Integration of HIV-1 viral DNA into the host genome is carried out by HIV-integrase (IN) and is a critical step in viral replication. Although several classes of compounds have been reported to inhibit IN in enzymatic assays, inhibition is not always correlated with antiviral activity. Moreover, potent antiviral IN inhibitors such as the chicoric acids do not act upon the intended enzymatic target but behave as entry inhibitors instead. The charged nature of the chicoric acids contributes to poor cellular uptake, and these compounds are further plagued by rapid ester hydrolysis in vivo. To address these critical deficiencies, we designed neutral, nonhydrolyzable analogues of the chicoric acids. Herein, we report the synthesis, enzyme inhibition studies, and cellular antiviral data for a series of geminal disulfones. Of the 10 compounds evaluated, 8 showed moderate to high inhibition of IN in purified enzyme assays. The purified enzyme data correlated with antiviral assays for all but two compounds, suggesting alternative modes of inhibition. Time-of-addition studies were performed on these analogues, and the results indicate that they inhibit an early stage in the replication process, perhaps entry. In contrast, the most potent member of the correlative group shows behavior consistent with IN being the cellular target.

Original languageEnglish (US)
Pages (from-to)4526-4534
Number of pages9
JournalJournal of Medicinal Chemistry
Volume48
Issue number14
DOIs
StatePublished - Jul 14 2005

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HIV Integrase Inhibitors
Integrase Inhibitors
Integrases
Antiviral Agents
Assays
Enzyme Assays
HIV Integrase
Enzyme inhibition
Viral DNA
Enzymes
HIV-1
Hydrolysis
Esters
Genes
Genome
p31 integrase protein, Human immunodeficiency virus 1
chicoric acid

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Meadows, D. C., Mathews, T. B., North, T. W., Hadd, M. J., Kuo, C. L., Neamati, N., & Gervay-Hague, J. (2005). Synthesis and biological evaluation of geminal disulfones as HIV-1 integrase inhibitors. Journal of Medicinal Chemistry, 48(14), 4526-4534. https://doi.org/10.1021/jm049171v

Synthesis and biological evaluation of geminal disulfones as HIV-1 integrase inhibitors. / Meadows, D. Christopher; Mathews, Timothy B.; North, Thomas W.; Hadd, Michael J.; Kuo, Chih Lin; Neamati, Nouri; Gervay-Hague, Jacquelyn.

In: Journal of Medicinal Chemistry, Vol. 48, No. 14, 14.07.2005, p. 4526-4534.

Research output: Contribution to journalArticle

Meadows DC, Mathews TB, North TW, Hadd MJ, Kuo CL, Neamati N et al. Synthesis and biological evaluation of geminal disulfones as HIV-1 integrase inhibitors. Journal of Medicinal Chemistry. 2005 Jul 14;48(14):4526-4534. https://doi.org/10.1021/jm049171v
Meadows, D. Christopher ; Mathews, Timothy B. ; North, Thomas W. ; Hadd, Michael J. ; Kuo, Chih Lin ; Neamati, Nouri ; Gervay-Hague, Jacquelyn. / Synthesis and biological evaluation of geminal disulfones as HIV-1 integrase inhibitors. In: Journal of Medicinal Chemistry. 2005 ; Vol. 48, No. 14. pp. 4526-4534.
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