Synovial stem cells and their responses to the porosity of microfibrous scaffold

Benjamin Li Ping Lee, Zhenyu Tang, Aijun Wang, Fang Huang, Zhiqiang Yan, Dong Wang, Julia S. Chu, Neerav Dixit, Li Yang, Song Li

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Tissue-specific stem cells can be coaxed or harvested for tissue regeneration. In this study, we identified and characterized a new type of stem cells from the synovial membrane of knee joint, named neural crest cell-like synovial stem cells (NCCL-SSCs). NCCL-SSCs showed the characteristics of neural crest stem cells: they expressed markers such as Sox10, Sox17 and S100β, were clonable, and could differentiate into neural lineages as well as mesenchymal lineages, although NCCL-SSCs were not derived from neural crest during the development. When treated with transforming growth factor β1 (TGF-β1), NCCL-SSCs differentiated into mesenchymal stem cells (MSCs), lost the expression of Sox17 and the differentiation potential into neural lineages, but retained the potential of differentiating into mesenchymal lineages. To determine the responses of NCCL-SSCs to microfibrous scaffolds for tissue engineering, electrospun composite scaffolds with various porosities were fabricated by co-electrospinning of structural and sacrificial microfibers. The increase in the porosity in microfibrous scaffolds enhanced cell infiltration in vitro and in vivo, but did not affect the morphology and the proliferation of NCCL-SSCs. Interestingly, microfibrous scaffolds with higher porosity increased the expression of chondrogenic and osteogenic genes but suppressed smooth muscle and adipogenic genes. These results suggest that the differentiation of NCCL-SSCs can be controlled by both soluble chemical factors and biophysical factors such as the porosity of the scaffold. Engineering both NCCL-SSCs and scaffolds will have tremendous potential for tissue regeneration.

Original languageEnglish (US)
Pages (from-to)7264-7275
Number of pages12
JournalActa Biomaterialia
Volume9
Issue number7
DOIs
StatePublished - Jul 2013

Fingerprint

Neural Crest
Porosity
Stem cells
Scaffolds
Stem Cells
Tissue regeneration
Scaffolds (biology)
Regeneration
Genes
Neural Stem Cells
Synovial Membrane
Transforming Growth Factors
Tissue Engineering
Knee Joint
Mesenchymal Stromal Cells
Electrospinning
Smooth Muscle
Tissue engineering
Infiltration
Cell Membrane

Keywords

  • Electrospinning
  • Neural crest
  • Porosity
  • Scaffold
  • Synovial stem cells

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Synovial stem cells and their responses to the porosity of microfibrous scaffold. / Lee, Benjamin Li Ping; Tang, Zhenyu; Wang, Aijun; Huang, Fang; Yan, Zhiqiang; Wang, Dong; Chu, Julia S.; Dixit, Neerav; Yang, Li; Li, Song.

In: Acta Biomaterialia, Vol. 9, No. 7, 07.2013, p. 7264-7275.

Research output: Contribution to journalArticle

Lee, BLP, Tang, Z, Wang, A, Huang, F, Yan, Z, Wang, D, Chu, JS, Dixit, N, Yang, L & Li, S 2013, 'Synovial stem cells and their responses to the porosity of microfibrous scaffold', Acta Biomaterialia, vol. 9, no. 7, pp. 7264-7275. https://doi.org/10.1016/j.actbio.2013.03.009
Lee, Benjamin Li Ping ; Tang, Zhenyu ; Wang, Aijun ; Huang, Fang ; Yan, Zhiqiang ; Wang, Dong ; Chu, Julia S. ; Dixit, Neerav ; Yang, Li ; Li, Song. / Synovial stem cells and their responses to the porosity of microfibrous scaffold. In: Acta Biomaterialia. 2013 ; Vol. 9, No. 7. pp. 7264-7275.
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