Syngeneic mouse mammary carcinoma cell lines: Two closely related cell lines with divergent metastatic behavior

Alexander D Borowsky, Ruria Namba, Lawrence J T Young, Kent W. Hunter, J. Graeme Hodgson, Clifford G Tepper, Erik T. McGoldrick, William J. Muller, Robert Cardiff, Jeffrey Gregg

Research output: Contribution to journalArticle

118 Scopus citations

Abstract

Two cell lines, Met-1fvb2 and DB-7fvb2, with different metastatic potential, were derived from mammary carcinomas in FVB/N-Tg(MMTV-PyVmT) and FVB/N-Tg(MMTV-PyVmT Y315F/Y322F ) mice, transplanted into syngeneic FVB/N hosts and characterized. The lines maintain a stable morphological and biological phenotype after multiple rounds of in vitro culture and in vivo transplantation. The Met-1fvb2 line derived from a FVB/N-Tg(MMTV-PyVmT) tumor exhibits invasive growth and 100% metastases when transplanted into the females FVB/N mammary fat pad. The DB-7fvb2 line derived from the FVB/N-Tg(MMTV-PyVmT Y315F/Y322F ) with a "double base" modification at Y315F/Y322F exhibits more rapid growth when transplanted into the mammary fat pad, but a lower rate of metastasis (17%). The Met1fvb2 cells show high activation of AKT, while DB-7fvb2 cells show very low levels of AKT activation. The DNA content and gene expression levels of both cell lines are stable over multiple generations. Therefore, these two cell lines provide a stable, reproducible resource for the study of metastasis modulators, AKT molecular pathway interactions, and gene target and marker discovery.

Original languageEnglish (US)
Pages (from-to)47-59
Number of pages13
JournalClinical and Experimental Metastasis
Volume22
Issue number1
DOIs
StatePublished - Jan 2005

Keywords

  • Akt
  • Breast
  • Breast carcinoma
  • Cell line metastasis
  • Comparative genomic hybridization
  • ERBB2
  • Gene expression analysis
  • LY294002
  • Mammary fat pad
  • Mouse mammary tumor virus long terminal repeat
  • Oncogene
  • Orthotopic
  • Phosphatidylinositol 3 kinase

ASJC Scopus subject areas

  • Cancer Research

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