Abstract
In previous studies we have shown interactions between ozone and acidic respirable aerosols as evaluated by sensitive biochemical and quantitative morphological endpoints. In the present paper we have attempted to test the hypothesis that active oxygen specis might play a role in lung damage caused by ozone ± sulfuric acid aerosol. Rats were administered various scavengers of active oxygen species via the intraperitoneal (i.p.) route before and during exposure to 0.12, 0.20 or 0.64 ppm of ozone ± 0.04, 0.1, 0.2, 0.5, or 1.0 mg/m3 of sulfuric acid aerosol. Vitamin E, a lipid-soluble free radical scavenger, β-carotene (the precursor of vitamin A), a singlet oxygen scavenger, and dimethylthiourea, a hydrogen peroxide scavenger, were tested. Dimethylthiourea at doses of 50 - 500 mg/kg per day was able to protect rats from the effets of ozone, suggesting a role for hydrogen peroxide in ozone-induced lung damage in vivo. Neither vitamin E nor β-carotene at 100 mg/kg doses had any protective activity in our experiments, despite documentation of increased lung content of the scavengers in animals receiving these agents. These results suggest that water solubility may play a role in the efficacy of the scavenging agents under our exposure protocols. We conclude that: (1) hydrogen peroxide may be involved in ozone-induced lung damage; and (2) that lung injury by ozone and by ozone + sulfuric acid aerosol may share common pathways.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 113-133 |
| Number of pages | 21 |
| Journal | Toxicology |
| Volume | 53 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 16 1988 |
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Keywords
- Air pollution
- Fibrosis
- Free radicals
- Ozone
ASJC Scopus subject areas
- Toxicology
Cite this
Synergistic interaction of ozone and respirable aerosols on rat lungs. IV. Protection by quenchers of reactive oxygen species. / Warren, Darren L.; Hyde, Dallas M.; Last, Jerold A.
In: Toxicology, Vol. 53, No. 1, 16.12.1988, p. 113-133.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synergistic interaction of ozone and respirable aerosols on rat lungs. IV. Protection by quenchers of reactive oxygen species
AU - Warren, Darren L.
AU - Hyde, Dallas M.
AU - Last, Jerold A
PY - 1988/12/16
Y1 - 1988/12/16
N2 - In previous studies we have shown interactions between ozone and acidic respirable aerosols as evaluated by sensitive biochemical and quantitative morphological endpoints. In the present paper we have attempted to test the hypothesis that active oxygen specis might play a role in lung damage caused by ozone ± sulfuric acid aerosol. Rats were administered various scavengers of active oxygen species via the intraperitoneal (i.p.) route before and during exposure to 0.12, 0.20 or 0.64 ppm of ozone ± 0.04, 0.1, 0.2, 0.5, or 1.0 mg/m3 of sulfuric acid aerosol. Vitamin E, a lipid-soluble free radical scavenger, β-carotene (the precursor of vitamin A), a singlet oxygen scavenger, and dimethylthiourea, a hydrogen peroxide scavenger, were tested. Dimethylthiourea at doses of 50 - 500 mg/kg per day was able to protect rats from the effets of ozone, suggesting a role for hydrogen peroxide in ozone-induced lung damage in vivo. Neither vitamin E nor β-carotene at 100 mg/kg doses had any protective activity in our experiments, despite documentation of increased lung content of the scavengers in animals receiving these agents. These results suggest that water solubility may play a role in the efficacy of the scavenging agents under our exposure protocols. We conclude that: (1) hydrogen peroxide may be involved in ozone-induced lung damage; and (2) that lung injury by ozone and by ozone + sulfuric acid aerosol may share common pathways.
AB - In previous studies we have shown interactions between ozone and acidic respirable aerosols as evaluated by sensitive biochemical and quantitative morphological endpoints. In the present paper we have attempted to test the hypothesis that active oxygen specis might play a role in lung damage caused by ozone ± sulfuric acid aerosol. Rats were administered various scavengers of active oxygen species via the intraperitoneal (i.p.) route before and during exposure to 0.12, 0.20 or 0.64 ppm of ozone ± 0.04, 0.1, 0.2, 0.5, or 1.0 mg/m3 of sulfuric acid aerosol. Vitamin E, a lipid-soluble free radical scavenger, β-carotene (the precursor of vitamin A), a singlet oxygen scavenger, and dimethylthiourea, a hydrogen peroxide scavenger, were tested. Dimethylthiourea at doses of 50 - 500 mg/kg per day was able to protect rats from the effets of ozone, suggesting a role for hydrogen peroxide in ozone-induced lung damage in vivo. Neither vitamin E nor β-carotene at 100 mg/kg doses had any protective activity in our experiments, despite documentation of increased lung content of the scavengers in animals receiving these agents. These results suggest that water solubility may play a role in the efficacy of the scavenging agents under our exposure protocols. We conclude that: (1) hydrogen peroxide may be involved in ozone-induced lung damage; and (2) that lung injury by ozone and by ozone + sulfuric acid aerosol may share common pathways.
KW - Air pollution
KW - Fibrosis
KW - Free radicals
KW - Ozone
UR - http://www.scopus.com/inward/record.url?scp=0024230402&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024230402&partnerID=8YFLogxK
U2 - 10.1016/0300-483X(88)90241-7
DO - 10.1016/0300-483X(88)90241-7
M3 - Article
C2 - 3144057
AN - SCOPUS:0024230402
VL - 53
SP - 113
EP - 133
JO - Toxicology
JF - Toxicology
SN - 0300-483X
IS - 1
ER -