Synergistic effects of lactic acid and sodium dodecyl sulfate to decontaminate Escherichia coli o157: h7 on cattle hide sections

Mohamed G. Elramady, Sharif S Aly, Paul V. Rossitto, Jennifer A. Crook, James S Cullor

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The objective of this study was to investigate the antibacterial properties of chitosan acetate (CA), sodium dodecyl sulfate (SDS), lactic acid (LA) and their synergism when combined against a nontoxigenic strain of Escherichia coli O157:H7. Treatments that significantly reduced the concentration of E. coli O157:H7 in vitro by more than two logs were further investigated using a cattle hide decontamination model. In vitro treatments included CA (1% chitosan in 1% acetic acid vol/vol), SDS (1% vol/vol), SDS (2% vol/vol), LA (1% vol/vol), CA-SDS combination (1% chitosan in 1% acetic acid vol/vol mixed with 1% SDS vol/vol), and LA-SDS combination in two different concentrations (1% LA mixed with 1% SDS vol/vol, and 1% LA mixed with 2% SDS vol/vol). Butterfield's Phosphate Buffer water was used as a control. The antibacterial effect of 1% CA solution alone and in combination with 1% SDS in vitro resulted in a 1.8 and 1.7 log colony-forming units (CFU)/mL reduction, respectively (p<0.05). Only 1% LA, 1% SDS, 2% SDS and their combinations resulted in a >2 log reduction in E. coli O157:H7. On hide sections, both 1% LA-1% SDS and 1% LA-2% SDS combinations significantly (p<0.05) reduced E. coli O157:H7 concentration by 4.6 and 4.7 log CFU/ cm2 greater than the control, respectively. There was no significant difference in the antibacterial effect of 1% LA compared to the control, 2% SDS compared to the control, or 1% LA compared to 2% SDS. Hence, the antibacterial efficacy of 1% LA against E. coli O157:H7 on hide sections was significantly enhanced when combined with 1% SDS. Results of this study support the use of low concentration LA-SDS combination as a hide wash to reduce the risk of E. coli O157:H7 contamination.

Original languageEnglish (US)
Pages (from-to)661-663
Number of pages3
JournalFoodborne Pathogens and Disease
Volume10
Issue number7
DOIs
StatePublished - Jul 1 2013

Fingerprint

Escherichia coli O157
sodium dodecyl sulfate
Sodium Dodecyl Sulfate
lactic acid
Lactic Acid
cattle
Chitosan
chitosan
Acetates
acetates
Acetic Acid
acetic acid
hides and skins
Stem Cells
nontoxigenic strains
Decontamination
antibacterial properties
decontamination
synergism

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Food Science
  • Animal Science and Zoology

Cite this

Synergistic effects of lactic acid and sodium dodecyl sulfate to decontaminate Escherichia coli o157 : h7 on cattle hide sections. / Elramady, Mohamed G.; Aly, Sharif S; Rossitto, Paul V.; Crook, Jennifer A.; Cullor, James S.

In: Foodborne Pathogens and Disease, Vol. 10, No. 7, 01.07.2013, p. 661-663.

Research output: Contribution to journalArticle

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abstract = "The objective of this study was to investigate the antibacterial properties of chitosan acetate (CA), sodium dodecyl sulfate (SDS), lactic acid (LA) and their synergism when combined against a nontoxigenic strain of Escherichia coli O157:H7. Treatments that significantly reduced the concentration of E. coli O157:H7 in vitro by more than two logs were further investigated using a cattle hide decontamination model. In vitro treatments included CA (1{\%} chitosan in 1{\%} acetic acid vol/vol), SDS (1{\%} vol/vol), SDS (2{\%} vol/vol), LA (1{\%} vol/vol), CA-SDS combination (1{\%} chitosan in 1{\%} acetic acid vol/vol mixed with 1{\%} SDS vol/vol), and LA-SDS combination in two different concentrations (1{\%} LA mixed with 1{\%} SDS vol/vol, and 1{\%} LA mixed with 2{\%} SDS vol/vol). Butterfield's Phosphate Buffer water was used as a control. The antibacterial effect of 1{\%} CA solution alone and in combination with 1{\%} SDS in vitro resulted in a 1.8 and 1.7 log colony-forming units (CFU)/mL reduction, respectively (p<0.05). Only 1{\%} LA, 1{\%} SDS, 2{\%} SDS and their combinations resulted in a >2 log reduction in E. coli O157:H7. On hide sections, both 1{\%} LA-1{\%} SDS and 1{\%} LA-2{\%} SDS combinations significantly (p<0.05) reduced E. coli O157:H7 concentration by 4.6 and 4.7 log CFU/ cm2 greater than the control, respectively. There was no significant difference in the antibacterial effect of 1{\%} LA compared to the control, 2{\%} SDS compared to the control, or 1{\%} LA compared to 2{\%} SDS. Hence, the antibacterial efficacy of 1{\%} LA against E. coli O157:H7 on hide sections was significantly enhanced when combined with 1{\%} SDS. Results of this study support the use of low concentration LA-SDS combination as a hide wash to reduce the risk of E. coli O157:H7 contamination.",
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