Synergistic actions of platelet‐derived growth factor and the insulin‐like growth factors in vivo: Enhancement of tissue repair in genetically diabetic mice

David G Greenhalgh, Robert P. Hummel, Steven Albertson, Matthew P. Breeden

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Topical application of growth factors has been shown to benefit both normal and impaired wound healing. In normal tissue repair, resident cells produce a “cocktail” of various types of growth factors that overlap in function. In vitro studies have proved that growth factor combinations can act synergistically to enhance cellular function beyond that achieved with individual growth factors. To determine whether similar combinations have a synergistic effect in vivo, we applied growth factor combinations topically to full‐thickness skin wounds created in genetically diabetic mice. The C57BL/KsJ‐db/db mouse is obese and has insulin‐resistant diabetes, and it has been proved that this mouse has markedly impaired wound healing. Topical application of platelet‐derived growth factor, insulin‐like growth factor‐I, or insulin‐like growth factor‐II enhances healing in this model. Marked synergism was found when platelet‐derived growth factor and insulin‐like growth factor‐II were combined to produce augmentation in wound closure beyond that achieved by application of the individual growth factors. The synergistic effect allowed for improved tissue repair at doses of platelet‐derived growth factor and insulin‐like growth factor‐II that were ineffective when applied individually. The addition of insulin‐like growth factor‐I or insulin to platelet‐derived growth factor produced no significant synergism. Because multiple growth factors are released in the wound during the healing process, it is not surprising that their combination further enhances healing. Growth factor combinations should become an important addition to the armamentarium for the treatment of chronic, nonhealing wounds.

Original languageEnglish (US)
Pages (from-to)69-81
Number of pages13
JournalWound Repair and Regeneration
Volume1
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Intercellular Signaling Peptides and Proteins
Wound Healing
Growth
Wounds and Injuries
Obese Mice
Insulin
Skin

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Synergistic actions of platelet‐derived growth factor and the insulin‐like growth factors in vivo : Enhancement of tissue repair in genetically diabetic mice. / Greenhalgh, David G; Hummel, Robert P.; Albertson, Steven; Breeden, Matthew P.

In: Wound Repair and Regeneration, Vol. 1, No. 2, 1993, p. 69-81.

Research output: Contribution to journalArticle

@article{3229fd49195e4799ba07f0be75abdf0d,
title = "Synergistic actions of platelet‐derived growth factor and the insulin‐like growth factors in vivo: Enhancement of tissue repair in genetically diabetic mice",
abstract = "Topical application of growth factors has been shown to benefit both normal and impaired wound healing. In normal tissue repair, resident cells produce a “cocktail” of various types of growth factors that overlap in function. In vitro studies have proved that growth factor combinations can act synergistically to enhance cellular function beyond that achieved with individual growth factors. To determine whether similar combinations have a synergistic effect in vivo, we applied growth factor combinations topically to full‐thickness skin wounds created in genetically diabetic mice. The C57BL/KsJ‐db/db mouse is obese and has insulin‐resistant diabetes, and it has been proved that this mouse has markedly impaired wound healing. Topical application of platelet‐derived growth factor, insulin‐like growth factor‐I, or insulin‐like growth factor‐II enhances healing in this model. Marked synergism was found when platelet‐derived growth factor and insulin‐like growth factor‐II were combined to produce augmentation in wound closure beyond that achieved by application of the individual growth factors. The synergistic effect allowed for improved tissue repair at doses of platelet‐derived growth factor and insulin‐like growth factor‐II that were ineffective when applied individually. The addition of insulin‐like growth factor‐I or insulin to platelet‐derived growth factor produced no significant synergism. Because multiple growth factors are released in the wound during the healing process, it is not surprising that their combination further enhances healing. Growth factor combinations should become an important addition to the armamentarium for the treatment of chronic, nonhealing wounds.",
author = "Greenhalgh, {David G} and Hummel, {Robert P.} and Steven Albertson and Breeden, {Matthew P.}",
year = "1993",
doi = "10.1046/j.1524-475X.1993.10206.x",
language = "English (US)",
volume = "1",
pages = "69--81",
journal = "Wound Repair and Regeneration",
issn = "1067-1927",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Synergistic actions of platelet‐derived growth factor and the insulin‐like growth factors in vivo

T2 - Enhancement of tissue repair in genetically diabetic mice

AU - Greenhalgh, David G

AU - Hummel, Robert P.

AU - Albertson, Steven

AU - Breeden, Matthew P.

PY - 1993

Y1 - 1993

N2 - Topical application of growth factors has been shown to benefit both normal and impaired wound healing. In normal tissue repair, resident cells produce a “cocktail” of various types of growth factors that overlap in function. In vitro studies have proved that growth factor combinations can act synergistically to enhance cellular function beyond that achieved with individual growth factors. To determine whether similar combinations have a synergistic effect in vivo, we applied growth factor combinations topically to full‐thickness skin wounds created in genetically diabetic mice. The C57BL/KsJ‐db/db mouse is obese and has insulin‐resistant diabetes, and it has been proved that this mouse has markedly impaired wound healing. Topical application of platelet‐derived growth factor, insulin‐like growth factor‐I, or insulin‐like growth factor‐II enhances healing in this model. Marked synergism was found when platelet‐derived growth factor and insulin‐like growth factor‐II were combined to produce augmentation in wound closure beyond that achieved by application of the individual growth factors. The synergistic effect allowed for improved tissue repair at doses of platelet‐derived growth factor and insulin‐like growth factor‐II that were ineffective when applied individually. The addition of insulin‐like growth factor‐I or insulin to platelet‐derived growth factor produced no significant synergism. Because multiple growth factors are released in the wound during the healing process, it is not surprising that their combination further enhances healing. Growth factor combinations should become an important addition to the armamentarium for the treatment of chronic, nonhealing wounds.

AB - Topical application of growth factors has been shown to benefit both normal and impaired wound healing. In normal tissue repair, resident cells produce a “cocktail” of various types of growth factors that overlap in function. In vitro studies have proved that growth factor combinations can act synergistically to enhance cellular function beyond that achieved with individual growth factors. To determine whether similar combinations have a synergistic effect in vivo, we applied growth factor combinations topically to full‐thickness skin wounds created in genetically diabetic mice. The C57BL/KsJ‐db/db mouse is obese and has insulin‐resistant diabetes, and it has been proved that this mouse has markedly impaired wound healing. Topical application of platelet‐derived growth factor, insulin‐like growth factor‐I, or insulin‐like growth factor‐II enhances healing in this model. Marked synergism was found when platelet‐derived growth factor and insulin‐like growth factor‐II were combined to produce augmentation in wound closure beyond that achieved by application of the individual growth factors. The synergistic effect allowed for improved tissue repair at doses of platelet‐derived growth factor and insulin‐like growth factor‐II that were ineffective when applied individually. The addition of insulin‐like growth factor‐I or insulin to platelet‐derived growth factor produced no significant synergism. Because multiple growth factors are released in the wound during the healing process, it is not surprising that their combination further enhances healing. Growth factor combinations should become an important addition to the armamentarium for the treatment of chronic, nonhealing wounds.

UR - http://www.scopus.com/inward/record.url?scp=84989052485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84989052485&partnerID=8YFLogxK

U2 - 10.1046/j.1524-475X.1993.10206.x

DO - 10.1046/j.1524-475X.1993.10206.x

M3 - Article

AN - SCOPUS:84989052485

VL - 1

SP - 69

EP - 81

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

SN - 1067-1927

IS - 2

ER -