SynDIG1: An Activity-Regulated, AMPA- Receptor-Interacting Transmembrane Protein that Regulates Excitatory Synapse Development

Evgenia Kalashnikova, Ramón A. Lorca, Inderpreet Kaur, Gustavo Barisone, Bonnie Li, Tatsuto Ishimaru, James Trimmer, Durga P. Mohapatra, Elva D Diaz

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

During development of the central nervous system, precise synaptic connections between presynaptic and postsynaptic neurons are formed. While significant progress has been made in our understanding of AMPA receptor trafficking during synaptic plasticity, less is known about the molecules that recruit AMPA receptors to nascent synapses during synaptogenesis. Here we identify a type II transmembrane protein (SynDIG1) that regulates AMPA receptor content at developing synapses in dissociated rat hippocampal neurons. SynDIG1 colocalizes with AMPA receptors at synapses and at extrasynaptic sites and associates with AMPA receptors in heterologous cells and brain. Altered levels of SynDIG1 in cultured neurons result in striking changes in excitatory synapse number and function. SynDIG1-mediated synapse development is dependent on association with AMPA receptors via its extracellular C terminus. Intriguingly, SynDIG1 content in dendritic spines is regulated by neuronal activity. Altogether, we define SynDIG1 as an activity-regulated transmembrane protein that regulates excitatory synapse development.

Original languageEnglish (US)
Pages (from-to)80-93
Number of pages14
JournalNeuron
Volume65
Issue number1
DOIs
StatePublished - Jan 14 2010

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Receptor-Interacting Protein Serine-Threonine Kinases
AMPA Receptors
Synapses
Neurons
Dendritic Spines
Neuronal Plasticity
Proteins
Central Nervous System
Brain

Keywords

  • CELLBIO
  • MOLNEURO
  • SIGNALING

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

SynDIG1 : An Activity-Regulated, AMPA- Receptor-Interacting Transmembrane Protein that Regulates Excitatory Synapse Development. / Kalashnikova, Evgenia; Lorca, Ramón A.; Kaur, Inderpreet; Barisone, Gustavo; Li, Bonnie; Ishimaru, Tatsuto; Trimmer, James; Mohapatra, Durga P.; Diaz, Elva D.

In: Neuron, Vol. 65, No. 1, 14.01.2010, p. 80-93.

Research output: Contribution to journalArticle

Kalashnikova, E, Lorca, RA, Kaur, I, Barisone, G, Li, B, Ishimaru, T, Trimmer, J, Mohapatra, DP & Diaz, ED 2010, 'SynDIG1: An Activity-Regulated, AMPA- Receptor-Interacting Transmembrane Protein that Regulates Excitatory Synapse Development', Neuron, vol. 65, no. 1, pp. 80-93. https://doi.org/10.1016/j.neuron.2009.12.021
Kalashnikova, Evgenia ; Lorca, Ramón A. ; Kaur, Inderpreet ; Barisone, Gustavo ; Li, Bonnie ; Ishimaru, Tatsuto ; Trimmer, James ; Mohapatra, Durga P. ; Diaz, Elva D. / SynDIG1 : An Activity-Regulated, AMPA- Receptor-Interacting Transmembrane Protein that Regulates Excitatory Synapse Development. In: Neuron. 2010 ; Vol. 65, No. 1. pp. 80-93.
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