Abstract
This article summarizes some of the recent advances in the understanding of structural and functional properties of isolated small synaptic vesicles (SSV) from mammalian brain. SSV contain a set of integral membrnae proteins which are highly specific for this organelle and which occur on all SSV of the central and peripheral nervous system irrespective of their transmitter content. In contrast, these proteins are absent from the membrane of peptide-containing large dense-core vesicles indicating that the two type of organelle have a different membrane composition. The availability of antibodies for these proteins has allowed the evaluation of the purity of vesicle preparations which is instrumental for functional studies. Recent advances in the study of neurotransmitter uptake have revealed that SSV contain specific carrier systems for glutamate and GABA. They are different from the transporters of the plasma membrane, and are dependent on the energy of a proton electrochemical gradient. The uptake of glutamate has been characterized in some detail and the mechanistic and physiological implications of these findings are discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 128-133 |
| Number of pages | 6 |
| Journal | Journal de Physiologie |
| Volume | 84 |
| Issue number | 1 |
| State | Published - 1990 |
| Externally published | Yes |
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Keywords
- Gaba
- Glutamate
- Neurotransmission
- Synaptic vesicles
ASJC Scopus subject areas
- Physiology
Cite this
Synaptic vesicles : Key organelles involved in neurotransmission. / Jahn, R.; Hell, Johannes W; Maycox, P. R.
In: Journal de Physiologie, Vol. 84, No. 1, 1990, p. 128-133.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synaptic vesicles
T2 - Key organelles involved in neurotransmission
AU - Jahn, R.
AU - Hell, Johannes W
AU - Maycox, P. R.
PY - 1990
Y1 - 1990
N2 - This article summarizes some of the recent advances in the understanding of structural and functional properties of isolated small synaptic vesicles (SSV) from mammalian brain. SSV contain a set of integral membrnae proteins which are highly specific for this organelle and which occur on all SSV of the central and peripheral nervous system irrespective of their transmitter content. In contrast, these proteins are absent from the membrane of peptide-containing large dense-core vesicles indicating that the two type of organelle have a different membrane composition. The availability of antibodies for these proteins has allowed the evaluation of the purity of vesicle preparations which is instrumental for functional studies. Recent advances in the study of neurotransmitter uptake have revealed that SSV contain specific carrier systems for glutamate and GABA. They are different from the transporters of the plasma membrane, and are dependent on the energy of a proton electrochemical gradient. The uptake of glutamate has been characterized in some detail and the mechanistic and physiological implications of these findings are discussed.
AB - This article summarizes some of the recent advances in the understanding of structural and functional properties of isolated small synaptic vesicles (SSV) from mammalian brain. SSV contain a set of integral membrnae proteins which are highly specific for this organelle and which occur on all SSV of the central and peripheral nervous system irrespective of their transmitter content. In contrast, these proteins are absent from the membrane of peptide-containing large dense-core vesicles indicating that the two type of organelle have a different membrane composition. The availability of antibodies for these proteins has allowed the evaluation of the purity of vesicle preparations which is instrumental for functional studies. Recent advances in the study of neurotransmitter uptake have revealed that SSV contain specific carrier systems for glutamate and GABA. They are different from the transporters of the plasma membrane, and are dependent on the energy of a proton electrochemical gradient. The uptake of glutamate has been characterized in some detail and the mechanistic and physiological implications of these findings are discussed.
KW - Gaba
KW - Glutamate
KW - Neurotransmission
KW - Synaptic vesicles
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UR - http://www.scopus.com/inward/citedby.url?scp=0025330805&partnerID=8YFLogxK
M3 - Article
C2 - 1972757
AN - SCOPUS:0025330805
VL - 84
SP - 128
EP - 133
JO - Journal de Physiologie
JF - Journal de Physiologie
SN - 0928-4257
IS - 1
ER -