Synaptic connections of dentate granule cells and hilar neurons: Results of paired intracellular recordings and intracellular horseradish peroxidase injections

H. E. Scharfman, D. D. Kunkel, Philip A Schwartzkroin

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

Simultaneous intracellular recordings were made in the dentate gyrus of rat hippocampal slices, from pairs of the following cell types: granule cells, interneurons located in the granule cell layer, hilar interneurons, and spiny hilar "mossy cells". Granule cells were found to have strong excitatory effects on mossy cells and interneurons. Interneurons inhibited granule cells as well as other interneurons. No synaptic connections from mossy cells onto other cell types were found, within the confines of the slice, using intracellular recording methods. However, at the ultrastructural level, axon terminals of horseradish peroxidase-filled mossy cells were found making synaptic contacts in the hilus on dendrites of interneurons. These studies provide the first step towards determining the functional interactions of the various cell types in the fascia dentata.

Original languageEnglish (US)
Pages (from-to)693-707
Number of pages15
JournalNeuroscience
Volume37
Issue number3
DOIs
StatePublished - 1990
Externally publishedYes

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Horseradish Peroxidase
Neurons
Interneurons
Injections
Dentate Gyrus
Presynaptic Terminals
Dendrites
Cell Communication

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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abstract = "Simultaneous intracellular recordings were made in the dentate gyrus of rat hippocampal slices, from pairs of the following cell types: granule cells, interneurons located in the granule cell layer, hilar interneurons, and spiny hilar {"}mossy cells{"}. Granule cells were found to have strong excitatory effects on mossy cells and interneurons. Interneurons inhibited granule cells as well as other interneurons. No synaptic connections from mossy cells onto other cell types were found, within the confines of the slice, using intracellular recording methods. However, at the ultrastructural level, axon terminals of horseradish peroxidase-filled mossy cells were found making synaptic contacts in the hilus on dendrites of interneurons. These studies provide the first step towards determining the functional interactions of the various cell types in the fascia dentata.",
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AU - Scharfman, H. E.

AU - Kunkel, D. D.

AU - Schwartzkroin, Philip A

PY - 1990

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