Sweat lipid mediator profiling: A noninvasive approach for cutaneous research

Karan Agrawal, Lauren A. Hassoun, Negar Foolad, Theresa L. Pedersen, Raja K Sivamani, John W. Newman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Recent advances in analytical and sweat collection techniques provide new opportunities to identify noninvasive biomarkers for the study of skin inflammation and repair. This study aims to characterize the lipid mediator profile including oxygenated lipids, endocannabinoids, and ceramides/ sphingoid bases in sweat and identify differences in these profiles between sweat collected from nonlesional sites on the unflared volar forearm of subjects with and without atopic dermatitis (AD). Adapting routine procedures developed for plasma analysis, over 100 lipid mediators were profiled using LC-MS/MS and 58 lipid mediators were detected in sweat. Lipid mediator concentrations were not affected by sampling or storage conditions. Increases in concentrations of C30-C40 [NS] and [NdS] ceramides, and C18:1 sphingosine, were observed in the sweat of study participants with AD despite no differences being observed in transepidermal water loss between study groups, and this effect was strongest in men (P < 0.05, one-way ANOVA with Tukey's post hoc HSD). No differences in oxylipins and endocannabinoids were observed between study groups. Sweat mediator profiling may therefore provide a noninvasive diagnostic for AD prior to the presentation of clinical signs.-Agrawal, K., L. A. Hassoun, N. Foolad, T. L. Pedersen, R. K. Sivamani, and J. W. Newman. Sweat lipid mediator profiling: a noninvasive approach for cutaneous research.

Original languageEnglish (US)
Pages (from-to)188-195
Number of pages8
JournalJournal of Lipid Research
Volume58
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Sweat
Lipids
Skin
Research
Atopic Dermatitis
Endocannabinoids
Ceramides
Oxylipins
Sphingosine
Biomarkers
Analysis of variance (ANOVA)
Forearm
Repair
Analysis of Variance
Sampling
Plasmas
Inflammation
Water

Keywords

  • Atopic dermatitis
  • Ceramides
  • Endocannabinoids
  • Metabolic profiling
  • Non-invasive sampling
  • Skin
  • Sphingolipids
  • Supplementary oxidized lipids

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Sweat lipid mediator profiling : A noninvasive approach for cutaneous research. / Agrawal, Karan; Hassoun, Lauren A.; Foolad, Negar; Pedersen, Theresa L.; Sivamani, Raja K; Newman, John W.

In: Journal of Lipid Research, Vol. 58, No. 1, 01.01.2017, p. 188-195.

Research output: Contribution to journalArticle

Agrawal, K, Hassoun, LA, Foolad, N, Pedersen, TL, Sivamani, RK & Newman, JW 2017, 'Sweat lipid mediator profiling: A noninvasive approach for cutaneous research', Journal of Lipid Research, vol. 58, no. 1, pp. 188-195. https://doi.org/10.1194/jlr.M071738
Agrawal, Karan ; Hassoun, Lauren A. ; Foolad, Negar ; Pedersen, Theresa L. ; Sivamani, Raja K ; Newman, John W. / Sweat lipid mediator profiling : A noninvasive approach for cutaneous research. In: Journal of Lipid Research. 2017 ; Vol. 58, No. 1. pp. 188-195.
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