Suz12 binds to silenced regions of the genome in a cell-type-specific manner

Sharon L. Squazzo, Henriette O'Geen, Vitalina M. Komashko, Sheryl R. Krig, Victor X. Jin, Sung Wook Jang, Raphael Margueron, Danny Reinberg, Roland Green, Peggy J. Farnham

Research output: Contribution to journalArticle

251 Citations (Scopus)

Abstract

Suz12 is a component of the Polycomb group complexes 2, 3, and 4 (PRC 2/3/4). These complexes are critical for proper embryonic development, but very few target genes have been identified in either mouse or human cells. Using a variety of ChIP-chip approaches, we have identified a large set of Suz12 target genes in five different human and mouse cell lines. Interestingly, we found that Suz12 target promoters are cell type specific, with transcription factors and homeobox proteins predominating in embryonal cells and glycoproteins and immunoglobulin-related proteins predominating in adult tumors. We have also characterized the localization of other components of the PRC complex with Suz12 and investigated the overall relationship between Suz12 binding and markers of active versus inactive chromatin, using both promoter arrays and custom tiling arrays. Surprisingly, we find that the PRC complexes can be localized to discrete binding sites or spread through large regions of the mouse and human genomes. Finally, we have shown that some Suz12 target genes are bound by OCT4 in embryonal cells and suggest that OCT4 maintains stem cell self-renewal, in part, by recruiting PRC complexes to certain genes that promote differentiation.

Original languageEnglish (US)
Pages (from-to)890-900
Number of pages11
JournalGenome Research
Volume16
Issue number7
DOIs
StatePublished - 2006

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Genome
Genes
Homeodomain Proteins
Human Genome
Chromatin
Embryonic Development
Immunoglobulins
Glycoproteins
Transcription Factors
Binding Sites
Cell Line
Neoplasms
Proteins
Cell Self Renewal

ASJC Scopus subject areas

  • Genetics

Cite this

Squazzo, S. L., O'Geen, H., Komashko, V. M., Krig, S. R., Jin, V. X., Jang, S. W., ... Farnham, P. J. (2006). Suz12 binds to silenced regions of the genome in a cell-type-specific manner. Genome Research, 16(7), 890-900. https://doi.org/10.1101/gr.5306606

Suz12 binds to silenced regions of the genome in a cell-type-specific manner. / Squazzo, Sharon L.; O'Geen, Henriette; Komashko, Vitalina M.; Krig, Sheryl R.; Jin, Victor X.; Jang, Sung Wook; Margueron, Raphael; Reinberg, Danny; Green, Roland; Farnham, Peggy J.

In: Genome Research, Vol. 16, No. 7, 2006, p. 890-900.

Research output: Contribution to journalArticle

Squazzo, SL, O'Geen, H, Komashko, VM, Krig, SR, Jin, VX, Jang, SW, Margueron, R, Reinberg, D, Green, R & Farnham, PJ 2006, 'Suz12 binds to silenced regions of the genome in a cell-type-specific manner', Genome Research, vol. 16, no. 7, pp. 890-900. https://doi.org/10.1101/gr.5306606
Squazzo SL, O'Geen H, Komashko VM, Krig SR, Jin VX, Jang SW et al. Suz12 binds to silenced regions of the genome in a cell-type-specific manner. Genome Research. 2006;16(7):890-900. https://doi.org/10.1101/gr.5306606
Squazzo, Sharon L. ; O'Geen, Henriette ; Komashko, Vitalina M. ; Krig, Sheryl R. ; Jin, Victor X. ; Jang, Sung Wook ; Margueron, Raphael ; Reinberg, Danny ; Green, Roland ; Farnham, Peggy J. / Suz12 binds to silenced regions of the genome in a cell-type-specific manner. In: Genome Research. 2006 ; Vol. 16, No. 7. pp. 890-900.
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