Susceptibility of whey protein isolate to oxidation and changes in physicochemical, structural, and digestibility characteristics

Xianchao Feng, Chenyi Li, Niamat Ullah, Jiqianrui Cao, Yongli Lan, Wupeng Ge, Robert M. Hackman, Zhixi Li, Lin Chen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Oxidation is an important factor for denaturing of whey protein isolate (WPI) during food processing. We studied the effects of chemical oxidation on physicochemical and structural changes along with in vitro digestibility of WPI in this work. Evaluation of physicochemical changes showed that carbonyl level and dityrosine content increased, whereas total and free thiol group levels decreased for oxidized WPI samples. For the structural changes, protein aggregation was measured by surface hydrophobicity, turbidity, and particle diameter, which was increased for oxidized WPI samples. The increase of the secondary structure β-sheets and antiparallel β-sheet also supported the aggregation of oxidized WPI. A direct quantitative relationship between physicochemical and structural changes and protein digestibility indicated that oxidation-related damage restricts the susceptibility of WPI to proteases. In conclusion, WPI had high susceptibility to oxidative stress, and both physicochemical and structural changes caused by severe oxidative stress could decrease the rate of in vitro digestibility of WPI.

Original languageEnglish (US)
Article number73889
Pages (from-to)7602-7613
Number of pages12
JournalJournal of Dairy Science
Volume98
Issue number11
DOIs
StatePublished - Nov 1 2015

Fingerprint

whey protein isolate
digestibility
oxidation
in vitro digestibility
Oxidative Stress
oxidative stress
Food Handling
Whey Proteins
hydrophobicity
digestible protein
thiols
Hydrophobic and Hydrophilic Interactions
food processing
Sulfhydryl Compounds
turbidity
Proteins
Peptide Hydrolases
proteinases
sampling

Keywords

  • Aggregation
  • In vitro digestion
  • Physicochemical changes
  • Structural changes
  • Whey protein isolates

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Science
  • Genetics

Cite this

Susceptibility of whey protein isolate to oxidation and changes in physicochemical, structural, and digestibility characteristics. / Feng, Xianchao; Li, Chenyi; Ullah, Niamat; Cao, Jiqianrui; Lan, Yongli; Ge, Wupeng; Hackman, Robert M.; Li, Zhixi; Chen, Lin.

In: Journal of Dairy Science, Vol. 98, No. 11, 73889, 01.11.2015, p. 7602-7613.

Research output: Contribution to journalArticle

Feng, X, Li, C, Ullah, N, Cao, J, Lan, Y, Ge, W, Hackman, RM, Li, Z & Chen, L 2015, 'Susceptibility of whey protein isolate to oxidation and changes in physicochemical, structural, and digestibility characteristics', Journal of Dairy Science, vol. 98, no. 11, 73889, pp. 7602-7613. https://doi.org/10.3168/jds.2015-9814
Feng, Xianchao ; Li, Chenyi ; Ullah, Niamat ; Cao, Jiqianrui ; Lan, Yongli ; Ge, Wupeng ; Hackman, Robert M. ; Li, Zhixi ; Chen, Lin. / Susceptibility of whey protein isolate to oxidation and changes in physicochemical, structural, and digestibility characteristics. In: Journal of Dairy Science. 2015 ; Vol. 98, No. 11. pp. 7602-7613.
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