Susceptibility of Culicoides variipennis sonorensis to infection by polymerase chain reaction-detectable bluetongue virus in cattle blood

Walter J. Tabachnick, Nigel J Maclachlan, Lee H. Thompson, Gregg J. Hunt, John F. Patton

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Cattle bloods containing only polymerase chain reaction (PCR)-detectable bluetongue-10 viral nucleic acid, but as determined by virus isolation techniques, not bluetongue-10 virus, were incapable of infecting intrathoracically inoculated Culicoides variipennis sonorensis. These insects also failed to transmit bluetongue-10 virus when fed on sheep. Cattle whose blood contain only PCR-detectable bluetongue viral nucleic acid, but no infectious virus, are unlikely to play a role in the epidemiology of bluetongue. The biological significance of PCR-based detection assays and their effect on animal health regulations on the international trade of livestock and livestock germplasm is discussed. Bluetongue virus infection provides a very useful model with which to study arthropod-transmitted RNA virus infections of humans and other animals.

Original languageEnglish (US)
Pages (from-to)481-485
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume54
Issue number5
StatePublished - May 1996

Fingerprint

Bluetongue
Ceratopogonidae
Bluetongue virus
Livestock
Polymerase Chain Reaction
Nucleic Acids
RNA Virus Infections
Infection
Viruses
Arthropods
Virus Diseases
Insects
Sheep
Epidemiology
Health

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Susceptibility of Culicoides variipennis sonorensis to infection by polymerase chain reaction-detectable bluetongue virus in cattle blood. / Tabachnick, Walter J.; Maclachlan, Nigel J; Thompson, Lee H.; Hunt, Gregg J.; Patton, John F.

In: American Journal of Tropical Medicine and Hygiene, Vol. 54, No. 5, 05.1996, p. 481-485.

Research output: Contribution to journalArticle

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