Survival rate of eukaryotic cells following electrophoretic nanoinjection

Matthias Simonis, Wolfgang Hübner, Alice Wilking, Thomas R Huser, Simon Hennig

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Insertion of foreign molecules such as functionalized fluorescent probes, antibodies, or plasmid DNA to living cells requires overcoming the plasma membrane barrier without harming the cell during the staining process. Many techniques such as electroporation, lipofection or microinjection have been developed to overcome the cellular plasma membrane, but they all result in reduced cell viability. A novel approach is the injection of cells with a nanopipette and using electrophoretic forces for the delivery of molecules. The tip size of these pipettes is approximately ten times smaller than typical microinjection pipettes and rather than pressure pulses as delivery method, moderate DC electric fields are used to drive charged molecules out of the tip. Here, we show that this approach leads to a significantly higher survival rate of nanoinjected cells and that injection with nanopipettes has a significantly lower impact on the proliferation behavior of injected cells. Thus, we propose that injection with nanopipettes using electrophoretic delivery is an excellent alternative when working with valuable and rare living cells, such as primary cells or stem cells.

Original languageEnglish (US)
Article number41277
JournalScientific Reports
Volume7
DOIs
StatePublished - Jan 25 2017

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Eukaryotic Cells
Microinjections
Injections
Cell Membrane
Electroporation
Fluorescent Dyes
Cell Survival
Plasmids
Stem Cells
Staining and Labeling
Blood Pressure
Antibodies
DNA

ASJC Scopus subject areas

  • General

Cite this

Simonis, M., Hübner, W., Wilking, A., Huser, T. R., & Hennig, S. (2017). Survival rate of eukaryotic cells following electrophoretic nanoinjection. Scientific Reports, 7, [41277]. https://doi.org/10.1038/srep41277

Survival rate of eukaryotic cells following electrophoretic nanoinjection. / Simonis, Matthias; Hübner, Wolfgang; Wilking, Alice; Huser, Thomas R; Hennig, Simon.

In: Scientific Reports, Vol. 7, 41277, 25.01.2017.

Research output: Contribution to journalArticle

Simonis, M, Hübner, W, Wilking, A, Huser, TR & Hennig, S 2017, 'Survival rate of eukaryotic cells following electrophoretic nanoinjection', Scientific Reports, vol. 7, 41277. https://doi.org/10.1038/srep41277
Simonis, Matthias ; Hübner, Wolfgang ; Wilking, Alice ; Huser, Thomas R ; Hennig, Simon. / Survival rate of eukaryotic cells following electrophoretic nanoinjection. In: Scientific Reports. 2017 ; Vol. 7.
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