Survival of sheep x goat hybrid inner cell masses after injection into ovine embryos

T. L. Roth, G. B. Anderson, Robert Bondurant, R. L. Pashen

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Modified blastocyst injection techniques were used to inject immunosurgically isolated sheep x goat hybrid inner cell masses (ICM) into ovine blastocysts, with subsequent transfer of composite embryos to ovine recipients. Hybrid embryos were collected from does artificially inseminated with Barbados ram semen. A total of 13 live and 2 aborted offspring resulted from the 34 composite embryos transferred to recipient ewes (38% embryo survival). Of the 15 offspring, 4 exhibited phenotypic hybridism and 2 (13%) of these were determined to be hybrid <-> sheep chimeras by karyotype, serum protein and isoenzyme analyses, and fiber identification. Each of the 4 was produced by an injection procedure that involved damage of the ovine host ICM. One additional offspring was unusual in appearance, but the presence of hybrid cells was not proven. Similarly, caprine ICM were immunosurgically isolated and injected into ovine blastocysts that were then transferred to ovine recipients. Of the 13 composite embryos transferred, 12 offspring were produced (92% embryo survival). Eleven were overt goat <-> sheep chimeras and, of these, 7 were also blood chimeras. The hybrid ICM was shown to be capable of contributing to normal embryonic and fetal development after injection into an ovine blastocyst but may be less likely to be incorporated with the ovine host ICM than is the caprine ICM.

Original languageEnglish (US)
Pages (from-to)675-682
Number of pages8
JournalBiology of Reproduction
Volume41
Issue number4
DOIs
StatePublished - Dec 1 1989

Fingerprint

Hybrid Cells
Goats
Sheep
Embryonic Structures
Injections
Blastocyst
Barbados
Embryonic and Fetal Development
Embryo Transfer
Semen
Karyotype
Isoenzymes
Blood Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Survival of sheep x goat hybrid inner cell masses after injection into ovine embryos. / Roth, T. L.; Anderson, G. B.; Bondurant, Robert; Pashen, R. L.

In: Biology of Reproduction, Vol. 41, No. 4, 01.12.1989, p. 675-682.

Research output: Contribution to journalArticle

Roth, T. L. ; Anderson, G. B. ; Bondurant, Robert ; Pashen, R. L. / Survival of sheep x goat hybrid inner cell masses after injection into ovine embryos. In: Biology of Reproduction. 1989 ; Vol. 41, No. 4. pp. 675-682.
@article{2674ad59a4db4c2bbe4f643b48d49742,
title = "Survival of sheep x goat hybrid inner cell masses after injection into ovine embryos",
abstract = "Modified blastocyst injection techniques were used to inject immunosurgically isolated sheep x goat hybrid inner cell masses (ICM) into ovine blastocysts, with subsequent transfer of composite embryos to ovine recipients. Hybrid embryos were collected from does artificially inseminated with Barbados ram semen. A total of 13 live and 2 aborted offspring resulted from the 34 composite embryos transferred to recipient ewes (38{\%} embryo survival). Of the 15 offspring, 4 exhibited phenotypic hybridism and 2 (13{\%}) of these were determined to be hybrid <-> sheep chimeras by karyotype, serum protein and isoenzyme analyses, and fiber identification. Each of the 4 was produced by an injection procedure that involved damage of the ovine host ICM. One additional offspring was unusual in appearance, but the presence of hybrid cells was not proven. Similarly, caprine ICM were immunosurgically isolated and injected into ovine blastocysts that were then transferred to ovine recipients. Of the 13 composite embryos transferred, 12 offspring were produced (92{\%} embryo survival). Eleven were overt goat <-> sheep chimeras and, of these, 7 were also blood chimeras. The hybrid ICM was shown to be capable of contributing to normal embryonic and fetal development after injection into an ovine blastocyst but may be less likely to be incorporated with the ovine host ICM than is the caprine ICM.",
author = "Roth, {T. L.} and Anderson, {G. B.} and Robert Bondurant and Pashen, {R. L.}",
year = "1989",
month = "12",
day = "1",
doi = "10.1095/biolreprod41.4.675",
language = "English (US)",
volume = "41",
pages = "675--682",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "4",

}

TY - JOUR

T1 - Survival of sheep x goat hybrid inner cell masses after injection into ovine embryos

AU - Roth, T. L.

AU - Anderson, G. B.

AU - Bondurant, Robert

AU - Pashen, R. L.

PY - 1989/12/1

Y1 - 1989/12/1

N2 - Modified blastocyst injection techniques were used to inject immunosurgically isolated sheep x goat hybrid inner cell masses (ICM) into ovine blastocysts, with subsequent transfer of composite embryos to ovine recipients. Hybrid embryos were collected from does artificially inseminated with Barbados ram semen. A total of 13 live and 2 aborted offspring resulted from the 34 composite embryos transferred to recipient ewes (38% embryo survival). Of the 15 offspring, 4 exhibited phenotypic hybridism and 2 (13%) of these were determined to be hybrid <-> sheep chimeras by karyotype, serum protein and isoenzyme analyses, and fiber identification. Each of the 4 was produced by an injection procedure that involved damage of the ovine host ICM. One additional offspring was unusual in appearance, but the presence of hybrid cells was not proven. Similarly, caprine ICM were immunosurgically isolated and injected into ovine blastocysts that were then transferred to ovine recipients. Of the 13 composite embryos transferred, 12 offspring were produced (92% embryo survival). Eleven were overt goat <-> sheep chimeras and, of these, 7 were also blood chimeras. The hybrid ICM was shown to be capable of contributing to normal embryonic and fetal development after injection into an ovine blastocyst but may be less likely to be incorporated with the ovine host ICM than is the caprine ICM.

AB - Modified blastocyst injection techniques were used to inject immunosurgically isolated sheep x goat hybrid inner cell masses (ICM) into ovine blastocysts, with subsequent transfer of composite embryos to ovine recipients. Hybrid embryos were collected from does artificially inseminated with Barbados ram semen. A total of 13 live and 2 aborted offspring resulted from the 34 composite embryos transferred to recipient ewes (38% embryo survival). Of the 15 offspring, 4 exhibited phenotypic hybridism and 2 (13%) of these were determined to be hybrid <-> sheep chimeras by karyotype, serum protein and isoenzyme analyses, and fiber identification. Each of the 4 was produced by an injection procedure that involved damage of the ovine host ICM. One additional offspring was unusual in appearance, but the presence of hybrid cells was not proven. Similarly, caprine ICM were immunosurgically isolated and injected into ovine blastocysts that were then transferred to ovine recipients. Of the 13 composite embryos transferred, 12 offspring were produced (92% embryo survival). Eleven were overt goat <-> sheep chimeras and, of these, 7 were also blood chimeras. The hybrid ICM was shown to be capable of contributing to normal embryonic and fetal development after injection into an ovine blastocyst but may be less likely to be incorporated with the ovine host ICM than is the caprine ICM.

UR - http://www.scopus.com/inward/record.url?scp=0024809067&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024809067&partnerID=8YFLogxK

U2 - 10.1095/biolreprod41.4.675

DO - 10.1095/biolreprod41.4.675

M3 - Article

C2 - 2620076

AN - SCOPUS:0024809067

VL - 41

SP - 675

EP - 682

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 4

ER -