Survival and cell cycle kinetics of human prostate cancer cell lines after single- and multifraction exposures to ionizing radiation

Susan L. Scott, Paul H. Gumerlock, Laurel A Beckett, Yueju Li, Zelanna Goldberg

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Purpose Fractionated radiation therapy is frequently used to treat prostate cancer with an underlying assumption that each daily dose of ionizing radiation (IR) results in equal cell killing. We used three human prostate cancer cell lines to evaluate how survival after a single 2-Gy dose may predict responses after daily repeated 2-Gy exposures. Methods and materials LNCaP, CWR22R, and PC3 cells were used in these studies. Survival after IR exposures was assessed using clonogenic assays and cell cycle responses were determined by flow cytometry. Results The experimentally determined multifraction survival differed significantly from that predicted from their single-dose SF2. LNCaP and CWR22R cells showed lower than predicted survivals; PC3 cells exhibited greater than predicted survival. Daily IR exposures resulted in changes in the cell cycle distributions beyond those caused by a single exposure to IR. Conclusions Our results show that in these prostate cancer cells: (1) survival after a clinically relevant dose of IR does not predict survival after multifraction IR, (2) cell cycle responses after a single 2 Gy dose can differ from those that occur when cells receive daily 2 Gy doses, and (3) some cell cycle changes that result from fractionated IR may predict their ultimate survival responses from such treatment.

Original languageEnglish (US)
Pages (from-to)219-227
Number of pages9
JournalInternational Journal of Radiation Oncology Biology Physics
Volume59
Issue number1
DOIs
StatePublished - May 1 2004

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Keywords

  • Cell cycle checkpoints
  • Fractionated radiotherapy
  • Ionizing radiation
  • Prostate cancer cells
  • Radiosensitivity

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation

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