Survival after transfer of "sexed" mouse embryos exposed to H-Y antisera

K. L. White, G. M. Lindner, G. B. Anderson, Robert Bondurant

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Immunological means were used to determine the sex of mouse embryos prior to transfer to pseudopregnant recipients. Antisera to histocompatibility-Y (H-Y) antigen were prepared in adult C57BL/6 female mice by repeated intraperitoneal injections of spleen cells from males of the same strain. Eight-to 16-cell embryos were cultured in BMOC-3 alone or BMOC-3 without bovine serum albumin to which one of the following had been added: H-Y antiserum and normal guinea pig serum (NGPS), NGPS alone, normal mouse serum alone or normal mouse serum and NGPS. After 24 hr of culture, embryos were classified as either affected or unaffected. An embryo was classified as affected if degeneration of the embryo or breakdown of one or more cells was observed. A total of 1000 embryos were cultured in BMOC-3 with H-Y antiserum and NGPS (treated embryos). Two hundred and fifty embryos were cultured in each of the other four media (control embryos). Eighty-seven (9%) of the control embryos and 479 (48%) of the treated embryos were classified as affected after culture. Unaffected embryos, approximately 12 each, were transferred to pseudopregnant recipients. One-hundred forty control embryos (17%) survived to term with 67 females (48%) and 73 males (52%) born. Fifty-eight treated embryos (14%) survived to term, producing 50 females (86%) and 8 males (14%). Percentage of females from embryos cultured in antiserum was greater than for embryos cultured in any other media (P<0.001). These results demonstrate that detection of H-Y antigen on preimplantation embryos may be a useful and effective method of determining sex of an embryo prior to transfer.

Original languageEnglish (US)
Pages (from-to)655-662
Number of pages8
JournalTheriogenology
Volume18
Issue number6
DOIs
StatePublished - Jan 1 1982

Fingerprint

Histocompatibility
blood serum
antiserum
Immune Sera
Embryonic Structures
guinea pigs
mice
antigens
Guinea Pigs
embryo culture
gender
Serum
bovine serum albumin
intraperitoneal injection
splenocytes
Histocompatibility Antigens
cells
Blastocyst
Bovine Serum Albumin

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Equine
  • Animal Science and Zoology

Cite this

Survival after transfer of "sexed" mouse embryos exposed to H-Y antisera. / White, K. L.; Lindner, G. M.; Anderson, G. B.; Bondurant, Robert.

In: Theriogenology, Vol. 18, No. 6, 01.01.1982, p. 655-662.

Research output: Contribution to journalArticle

White, K. L. ; Lindner, G. M. ; Anderson, G. B. ; Bondurant, Robert. / Survival after transfer of "sexed" mouse embryos exposed to H-Y antisera. In: Theriogenology. 1982 ; Vol. 18, No. 6. pp. 655-662.
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