Survey of ground beef for the detection of Mycobacterium avium paratuberculosis

Carmela V. Jaravata, Wayne L. Smith, Gabriel J. Rensen, Juliana Ruzante, James S Cullor

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Mycobacterium avium paratuberculosis (MAP) is thought to be associated with Crohn's disease in humans. Since Johne's disease affects dairy and beef cattle, meat may be a possible route of transmission of MAP to humans. In this study, we compared a rapid multiplex real time PCR assay and conventional culture to detect MAP in ground beef. The real time PCR assay amplifies both an internal sequence of the IS900 gene and an internal control targeting the ruminant-specific mt-cyt-b gene, in order to control for any false negative results. The sensitivity of this multiplex real time PCR assay on ground beef is 101 CFU/g and the sensitivity of conventional culture at 10 3 CFU/g. Furthermore, we conducted a survey of 200 retail ground beef samples using this system and did not detect the presence of MAP.

Original languageEnglish (US)
Pages (from-to)103-106
Number of pages4
JournalFoodborne Pathogens and Disease
Volume4
Issue number1
DOIs
StatePublished - Mar 2007

Fingerprint

Mycobacterium avium subsp. paratuberculosis
Mycobacterium avium
ground beef
paratuberculosis
Real-Time Polymerase Chain Reaction
Multiplex Polymerase Chain Reaction
quantitative polymerase chain reaction
Paratuberculosis
assays
Gene Order
Ruminants
Crohn Disease
Crohn disease
Meat
beef cattle
dairy cattle
ruminants
Surveys and Questionnaires
Red Meat
meat

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Infectious Diseases
  • Food Science

Cite this

Survey of ground beef for the detection of Mycobacterium avium paratuberculosis. / Jaravata, Carmela V.; Smith, Wayne L.; Rensen, Gabriel J.; Ruzante, Juliana; Cullor, James S.

In: Foodborne Pathogens and Disease, Vol. 4, No. 1, 03.2007, p. 103-106.

Research output: Contribution to journalArticle

Jaravata, Carmela V. ; Smith, Wayne L. ; Rensen, Gabriel J. ; Ruzante, Juliana ; Cullor, James S. / Survey of ground beef for the detection of Mycobacterium avium paratuberculosis. In: Foodborne Pathogens and Disease. 2007 ; Vol. 4, No. 1. pp. 103-106.
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