Abstract
Mycobacterium avium paratuberculosis (MAP) is thought to be associated with Crohn's disease in humans. Since Johne's disease affects dairy and beef cattle, meat may be a possible route of transmission of MAP to humans. In this study, we compared a rapid multiplex real time PCR assay and conventional culture to detect MAP in ground beef. The real time PCR assay amplifies both an internal sequence of the IS900 gene and an internal control targeting the ruminant-specific mt-cyt-b gene, in order to control for any false negative results. The sensitivity of this multiplex real time PCR assay on ground beef is 101 CFU/g and the sensitivity of conventional culture at 10 3 CFU/g. Furthermore, we conducted a survey of 200 retail ground beef samples using this system and did not detect the presence of MAP.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 103-106 |
| Number of pages | 4 |
| Journal | Foodborne Pathogens and Disease |
| Volume | 4 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 2007 |
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ASJC Scopus subject areas
- Microbiology
- Applied Microbiology and Biotechnology
- Infectious Diseases
- Food Science
Cite this
Survey of ground beef for the detection of Mycobacterium avium paratuberculosis. / Jaravata, Carmela V.; Smith, Wayne L.; Rensen, Gabriel J.; Ruzante, Juliana; Cullor, James S.
In: Foodborne Pathogens and Disease, Vol. 4, No. 1, 03.2007, p. 103-106.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Survey of ground beef for the detection of Mycobacterium avium paratuberculosis
AU - Jaravata, Carmela V.
AU - Smith, Wayne L.
AU - Rensen, Gabriel J.
AU - Ruzante, Juliana
AU - Cullor, James S
PY - 2007/3
Y1 - 2007/3
N2 - Mycobacterium avium paratuberculosis (MAP) is thought to be associated with Crohn's disease in humans. Since Johne's disease affects dairy and beef cattle, meat may be a possible route of transmission of MAP to humans. In this study, we compared a rapid multiplex real time PCR assay and conventional culture to detect MAP in ground beef. The real time PCR assay amplifies both an internal sequence of the IS900 gene and an internal control targeting the ruminant-specific mt-cyt-b gene, in order to control for any false negative results. The sensitivity of this multiplex real time PCR assay on ground beef is 101 CFU/g and the sensitivity of conventional culture at 10 3 CFU/g. Furthermore, we conducted a survey of 200 retail ground beef samples using this system and did not detect the presence of MAP.
AB - Mycobacterium avium paratuberculosis (MAP) is thought to be associated with Crohn's disease in humans. Since Johne's disease affects dairy and beef cattle, meat may be a possible route of transmission of MAP to humans. In this study, we compared a rapid multiplex real time PCR assay and conventional culture to detect MAP in ground beef. The real time PCR assay amplifies both an internal sequence of the IS900 gene and an internal control targeting the ruminant-specific mt-cyt-b gene, in order to control for any false negative results. The sensitivity of this multiplex real time PCR assay on ground beef is 101 CFU/g and the sensitivity of conventional culture at 10 3 CFU/g. Furthermore, we conducted a survey of 200 retail ground beef samples using this system and did not detect the presence of MAP.
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UR - http://www.scopus.com/inward/citedby.url?scp=33947503863&partnerID=8YFLogxK
U2 - 10.1089/fpd.2006.54
DO - 10.1089/fpd.2006.54
M3 - Article
C2 - 17378715
AN - SCOPUS:33947503863
VL - 4
SP - 103
EP - 106
JO - Foodborne Pathogens and Disease
JF - Foodborne Pathogens and Disease
SN - 1535-3141
IS - 1
ER -