Surveillance for highly pathogenic influenza A viruses in California during 2014-2015 provides insights into viral evolutionary pathways and the spatiotemporal extent of viruses in the Pacific Americas Flyway

Andrew M. Ramey, Nichola J. Hill, Troy Cline, Magdalena Plancarte, Susan De La Cruz, Michael L. Casazza, Joshua T. Ackerman, Joseph P. Fleskes, T. Winston Vickers, Andrew B. Reeves, Frances Gulland, Christine Fontaine, Diann J. Prosser, Jonathan A. Runstadler, Walter M Boyce

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We used surveillance data collected in California before, concurrent with, and subsequent to an outbreak of highly pathogenic (HP) clade 2.3.4.4 influenza A viruses (IAVs) in 2014-2015 to (i) evaluate IAV prevalence in waterfowl, (ii) assess the evidence for spill-over infections in marine mammals and (iii) genetically characterize low-pathogenic (LP) and HP IAVs to refine inference on the spatiotemporal extent of HP genome constellations and to evaluate possible evolutionary pathways. We screened samples from 1496 waterfowl and 1142 marine mammals collected from April 2014 to August 2015 and detected IAV RNA in 159 samples collected from birds (n=157) and pinnipeds (n=2). HP IAV RNA was identified in three samples originating from American wigeon (Anas americana). Genetic sequence data were generated for a clade 2.3.4.4 HP IAV-positive diagnostic sample and 57 LP IAV isolates. Phylogenetic analyses revealed that the HP IAV was a reassortant H5N8 virus with gene segments closely related to LP IAVs detected in mallards (Anas platyrhynchos) sampled in California and other IAVs detected in wild birds sampled within the Pacific Americas Flyway. In addition, our analysis provided support for common ancestry between LP IAVs recovered from waterfowl sampled in California and gene segments of reassortant HP H5N1 IAVs detected in British Columbia, Canada and Washington, USA. Our investigation provides evidence that waterfowl are likely to have played a role in the evolution of reassortant HP IAVs in the Pacific Americas Flyway during 2014-2015, whereas we did not find support for spill-over infections in potential pinniped hosts.

Original languageEnglish (US)
Article numbere80
JournalEmerging Microbes and Infections
Volume6
Issue number9
DOIs
StatePublished - Sep 6 2017

Fingerprint

Influenza A virus
Viruses
Pinnipedia
Birds
Mammals
Reassortant Viruses
RNA
H5N1 Subtype Influenza A Virus
British Columbia
Infection
Genes
Canada
Disease Outbreaks

Keywords

  • California
  • clade 2.3.4.4
  • H5
  • highly pathogenic
  • influenza A
  • pinniped
  • virus
  • waterfowl

ASJC Scopus subject areas

  • Parasitology
  • Epidemiology
  • Microbiology
  • Immunology
  • Drug Discovery
  • Infectious Diseases
  • Virology

Cite this

Surveillance for highly pathogenic influenza A viruses in California during 2014-2015 provides insights into viral evolutionary pathways and the spatiotemporal extent of viruses in the Pacific Americas Flyway. / Ramey, Andrew M.; Hill, Nichola J.; Cline, Troy; Plancarte, Magdalena; De La Cruz, Susan; Casazza, Michael L.; Ackerman, Joshua T.; Fleskes, Joseph P.; Vickers, T. Winston; Reeves, Andrew B.; Gulland, Frances; Fontaine, Christine; Prosser, Diann J.; Runstadler, Jonathan A.; Boyce, Walter M.

In: Emerging Microbes and Infections, Vol. 6, No. 9, e80, 06.09.2017.

Research output: Contribution to journalArticle

Ramey, AM, Hill, NJ, Cline, T, Plancarte, M, De La Cruz, S, Casazza, ML, Ackerman, JT, Fleskes, JP, Vickers, TW, Reeves, AB, Gulland, F, Fontaine, C, Prosser, DJ, Runstadler, JA & Boyce, WM 2017, 'Surveillance for highly pathogenic influenza A viruses in California during 2014-2015 provides insights into viral evolutionary pathways and the spatiotemporal extent of viruses in the Pacific Americas Flyway', Emerging Microbes and Infections, vol. 6, no. 9, e80. https://doi.org/10.1038/emi.2017.66
Ramey, Andrew M. ; Hill, Nichola J. ; Cline, Troy ; Plancarte, Magdalena ; De La Cruz, Susan ; Casazza, Michael L. ; Ackerman, Joshua T. ; Fleskes, Joseph P. ; Vickers, T. Winston ; Reeves, Andrew B. ; Gulland, Frances ; Fontaine, Christine ; Prosser, Diann J. ; Runstadler, Jonathan A. ; Boyce, Walter M. / Surveillance for highly pathogenic influenza A viruses in California during 2014-2015 provides insights into viral evolutionary pathways and the spatiotemporal extent of viruses in the Pacific Americas Flyway. In: Emerging Microbes and Infections. 2017 ; Vol. 6, No. 9.
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abstract = "We used surveillance data collected in California before, concurrent with, and subsequent to an outbreak of highly pathogenic (HP) clade 2.3.4.4 influenza A viruses (IAVs) in 2014-2015 to (i) evaluate IAV prevalence in waterfowl, (ii) assess the evidence for spill-over infections in marine mammals and (iii) genetically characterize low-pathogenic (LP) and HP IAVs to refine inference on the spatiotemporal extent of HP genome constellations and to evaluate possible evolutionary pathways. We screened samples from 1496 waterfowl and 1142 marine mammals collected from April 2014 to August 2015 and detected IAV RNA in 159 samples collected from birds (n=157) and pinnipeds (n=2). HP IAV RNA was identified in three samples originating from American wigeon (Anas americana). Genetic sequence data were generated for a clade 2.3.4.4 HP IAV-positive diagnostic sample and 57 LP IAV isolates. Phylogenetic analyses revealed that the HP IAV was a reassortant H5N8 virus with gene segments closely related to LP IAVs detected in mallards (Anas platyrhynchos) sampled in California and other IAVs detected in wild birds sampled within the Pacific Americas Flyway. In addition, our analysis provided support for common ancestry between LP IAVs recovered from waterfowl sampled in California and gene segments of reassortant HP H5N1 IAVs detected in British Columbia, Canada and Washington, USA. Our investigation provides evidence that waterfowl are likely to have played a role in the evolution of reassortant HP IAVs in the Pacific Americas Flyway during 2014-2015, whereas we did not find support for spill-over infections in potential pinniped hosts.",
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