Surfactant protein D deficiency influences allergic immune responses

B. Schaub, R. M. Westlake, H. He, R. Arestides, K. J. Haley, M. Campo, G. Velasco, A. Bellou, S. Hawgood, Francis R Poulain, D. L. Perkins, Patricia W. Finn

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Background: The collectin surfactant protein D (SP-D) confers protection against pulmonary infection and inflammation. Recent data suggest a role for SP-D in the modulation of allergic inflammation. Objective: The aim of this study is to characterize the immune responses of SP-D-deficient (SP-D -/-) mice in a kinetic model of allergic inflammation. We determined whether allergic parameters were enhanced in SP-D-/- mice in vivo. Further, we examined whether functional immune responses in vitro such as lymphocyte proliferation (LP) and cytokine production were modulated in the absence of SP-D. Methods: In vivo, wild-type (WT) and SP-D-/- mice were sensitized and challenged with the allergen ovalbumin (OVA) and assessed for allergic parameters (bronchoalveolar lavage (BAL) eosinophils, IL-13 production, pulmonary IFN-γ, IL-10 expression) at early time points (1 and 3 days of challenge) in comparison with late time points (7 days of challenge). In vitro, spleen cells from WT and SP-D-/- mice were stimulated with the mitogen concanavalin A (ConA) and lipid A (LpA) and analysed for LP, IL-13 and IFN-γ production. Toll-like receptor 4 (TLR4), ligand for LpA, was assessed by mRNA expression and immunohistochemistry in vivo. Results: Following allergen exposure in vivo, SP-D-/- mice expressed higher BAL eosinophils and IL-13 concentrations and lower IFN-γ expression at early time points compared with WT mice. IL-10 expression was increased at early time points in SP-D-/- compared with WT mice. Allergen-induced TLR4 expression was increased in WT, but not in SP-D-/- mice. After stimulation with LpA and ConA in vitro LP was increased and IFN-γ concentration was decreased in SP-D-/- mice. Conclusion: SP-D may be critical for the modulation of early stages of allergic inflammation in vivo.

Original languageEnglish (US)
Pages (from-to)1819-1826
Number of pages8
JournalClinical and Experimental Allergy
Volume34
Issue number12
DOIs
StatePublished - Dec 2004
Externally publishedYes

Keywords

  • Allergy
  • Cytokines
  • Inflammation
  • Lung
  • SP-D
  • TLR4

ASJC Scopus subject areas

  • Immunology

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