Surfactant copolymers prevent aggregation of heat denatured lysozyme

Raphael C. Lee, Florin Despa, L. Guo, Pravin Betala, Anne Kuo, P. Thiyagarajan

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We investigated the ability of certain triblock copolymer surfactant poloxamers of the form polyethylene oxide-polypropylene oxide-polyethylene oxide (PEO-PPO-PEO), to prevent formation of stable aggregates of heat denatured hen egg lysozyme. Differential scanning calorimetry (DSC) and synchrotron small angle x-ray scattering (SAXS) experiments were performed to study the thermodynamics and solution structures of lysozyme at temperatures between 20 and 90°C in the presence and absence of poloxamers with various molecular weights (8.4-14.3 kDa), but similar hydrophile/hydrophobe (PEO:PPO) ratio of 80%. Poloxmer 188 was found to be very effective in preventing aggregation of heat denatured lysozyme and those functioned as a synthetic surfactant, thus enabling them to refold when the conditions become optimal. For comparison, we measured the ability of 8 kDa polyethylene glycol (PEG) to prevent lysozyme aggregation under same conditions. The results of these studies suggest that poloxamers are more efficient than PEG in preventing aggregation of heat denaturated lysozyme, To achieve equivalence, more than an order of magnitude higher concentration of PEG concentration was needed. Apparently, the presence of a hydrophobic segment in the poloxamers increases their ability to target the hydrophobic region of the unfolded proteins and protect them from self association. Given their biocompatibility and the low concentrations at which they effectively facilitate refolding of denatured proteins, they may be useful in the treatment of burns and other conditions resulting in the denaturation of proteins.

Original languageEnglish (US)
Pages (from-to)1190-1200
Number of pages11
JournalAnnals of Biomedical Engineering
Volume34
Issue number7
DOIs
StatePublished - Jul 2006
Externally publishedYes

Fingerprint

Surface active agents
Agglomeration
Copolymers
Enzymes
Polyethylene oxides
Polyethylene glycols
Proteins
Polypropylene oxides
Polyphenylene oxides
Denaturation
Synchrotrons
Biocompatibility
Block copolymers
Differential scanning calorimetry
Molecular weight
Hot Temperature
Association reactions
Thermodynamics
Scattering
X rays

Keywords

  • DSC
  • Heat denatured proteins
  • Poloxamer
  • Protein refolding
  • SAXS
  • Surfactants

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Lee, R. C., Despa, F., Guo, L., Betala, P., Kuo, A., & Thiyagarajan, P. (2006). Surfactant copolymers prevent aggregation of heat denatured lysozyme. Annals of Biomedical Engineering, 34(7), 1190-1200. https://doi.org/10.1007/s10439-006-9139-z

Surfactant copolymers prevent aggregation of heat denatured lysozyme. / Lee, Raphael C.; Despa, Florin; Guo, L.; Betala, Pravin; Kuo, Anne; Thiyagarajan, P.

In: Annals of Biomedical Engineering, Vol. 34, No. 7, 07.2006, p. 1190-1200.

Research output: Contribution to journalArticle

Lee, RC, Despa, F, Guo, L, Betala, P, Kuo, A & Thiyagarajan, P 2006, 'Surfactant copolymers prevent aggregation of heat denatured lysozyme', Annals of Biomedical Engineering, vol. 34, no. 7, pp. 1190-1200. https://doi.org/10.1007/s10439-006-9139-z
Lee, Raphael C. ; Despa, Florin ; Guo, L. ; Betala, Pravin ; Kuo, Anne ; Thiyagarajan, P. / Surfactant copolymers prevent aggregation of heat denatured lysozyme. In: Annals of Biomedical Engineering. 2006 ; Vol. 34, No. 7. pp. 1190-1200.
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