Abstract
In order to determine if the Adult Respiratory Distress Syndrome (ARDS) is associated with a shortage of pulmonary surfactant, we have examined a series of 155 undiluted trachea aspirates obtained from 23 patients with ARDS and from 30 patients without ARDS, all 53 needing ventilatory support. The unfixed and unstained specimens were examined by polarized light microscopy for the presence of pulmonary surfactant. Free surfactant particles were present in the aspirates of 50 patients (95%). Within minutes of obtaining the specimen, in addition to showing surfactant, the examination of these undiluted, unfixed trachea aspirates by light microscopy showed the presence of bacterial and fungal infection. As fibrinogenj fibrin inactivates surfactant, the undiluted aspirates of 21 patients with ARDS and of 30 patients without ARDS were stained for the presence of these proteins by a new slide method using 0.01 ml of trachea aspirate. Fibrinogen/fibrin was found in the aspirates from 20 of 21 patients with ARDS and in only five of 30 patients without ARDS; these five had bronchopneumonia. The method has a sensitivity of 0.95 and a specificity of 0.83. The role of plasma proteins and of neutrophil leucocytes in causing ARDS is discussed. (Br. J. Anaesth. 1993; 70: 522-526).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 522-526 |
| Number of pages | 5 |
| Journal | British Journal of Anaesthesia |
| Volume | 70 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 1993 |
| Externally published | Yes |
Fingerprint
Keywords
- Lung: adult respiratory distress syndrome
- Surfactant
ASJC Scopus subject areas
- Anesthesiology and Pain Medicine
Cite this
Surfactant and adult respiratory distress syndrome. / Jacobson, W.; Park, G. R.; Saich, T.; Park, G. R.; Holcroft, James W.
In: British Journal of Anaesthesia, Vol. 70, No. 5, 01.01.1993, p. 522-526.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Surfactant and adult respiratory distress syndrome
AU - Jacobson, W.
AU - Park, G. R.
AU - Saich, T.
AU - Park, G. R.
AU - Holcroft, James W
PY - 1993/1/1
Y1 - 1993/1/1
N2 - In order to determine if the Adult Respiratory Distress Syndrome (ARDS) is associated with a shortage of pulmonary surfactant, we have examined a series of 155 undiluted trachea aspirates obtained from 23 patients with ARDS and from 30 patients without ARDS, all 53 needing ventilatory support. The unfixed and unstained specimens were examined by polarized light microscopy for the presence of pulmonary surfactant. Free surfactant particles were present in the aspirates of 50 patients (95%). Within minutes of obtaining the specimen, in addition to showing surfactant, the examination of these undiluted, unfixed trachea aspirates by light microscopy showed the presence of bacterial and fungal infection. As fibrinogenj fibrin inactivates surfactant, the undiluted aspirates of 21 patients with ARDS and of 30 patients without ARDS were stained for the presence of these proteins by a new slide method using 0.01 ml of trachea aspirate. Fibrinogen/fibrin was found in the aspirates from 20 of 21 patients with ARDS and in only five of 30 patients without ARDS; these five had bronchopneumonia. The method has a sensitivity of 0.95 and a specificity of 0.83. The role of plasma proteins and of neutrophil leucocytes in causing ARDS is discussed. (Br. J. Anaesth. 1993; 70: 522-526).
AB - In order to determine if the Adult Respiratory Distress Syndrome (ARDS) is associated with a shortage of pulmonary surfactant, we have examined a series of 155 undiluted trachea aspirates obtained from 23 patients with ARDS and from 30 patients without ARDS, all 53 needing ventilatory support. The unfixed and unstained specimens were examined by polarized light microscopy for the presence of pulmonary surfactant. Free surfactant particles were present in the aspirates of 50 patients (95%). Within minutes of obtaining the specimen, in addition to showing surfactant, the examination of these undiluted, unfixed trachea aspirates by light microscopy showed the presence of bacterial and fungal infection. As fibrinogenj fibrin inactivates surfactant, the undiluted aspirates of 21 patients with ARDS and of 30 patients without ARDS were stained for the presence of these proteins by a new slide method using 0.01 ml of trachea aspirate. Fibrinogen/fibrin was found in the aspirates from 20 of 21 patients with ARDS and in only five of 30 patients without ARDS; these five had bronchopneumonia. The method has a sensitivity of 0.95 and a specificity of 0.83. The role of plasma proteins and of neutrophil leucocytes in causing ARDS is discussed. (Br. J. Anaesth. 1993; 70: 522-526).
KW - Lung: adult respiratory distress syndrome
KW - Surfactant
UR - http://www.scopus.com/inward/record.url?scp=0027196698&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027196698&partnerID=8YFLogxK
U2 - 10.1093/bja/70.5.522
DO - 10.1093/bja/70.5.522
M3 - Article
C2 - 8318324
AN - SCOPUS:0027196698
VL - 70
SP - 522
EP - 526
JO - British Journal of Anaesthesia
JF - British Journal of Anaesthesia
SN - 0007-0912
IS - 5
ER -