Abstract
A series of composite films were prepared by blending chitosan with three polycations, poly(L-lysine), polyethyleneimine, and poly-L-ornithine, in specific blend proportions. The surface properties of the composite films, including surface topography, chemistry, and wettability, were examined by atomic force microscopy, X-ray photoelectron spectroscopy, and contact angle assay, respectively. For all composite films, blending with different polycations produced different nanoscaled surface topographical features (particles, granules, fibers, and islands) in addition to inducing changes in surface chemistry and wettability. PC12 cells were cultured on these composite films to evaluate the effects of surface properties on cell behavior. The PC12 cell behavior was holistically affected by surface topography, chemistry, and wettability; the cells also displayed responses to surface topography. On the surfaces with fiber topographic features, the PC12 cells exhibited significantly higher levels of adhesion, proliferation, and differentiation when compared to particle, granule, or island dominant surfaces. It appears that the surface topography of chitosan and chitosan-derived materials may play an important role in regulating nerve cell behavior and that topographic modification can be utilized for the applications of chitosan and chitosan-derived materials in nerve or other tissue regeneration.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 63-82 |
| Number of pages | 20 |
| Journal | Journal of Bioactive and Compatible Polymers |
| Volume | 24 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2009 |
| Externally published | Yes |
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Keywords
- AFM
- Cell behavior
- Chitosan
- Chitosan blends
- Chitosan-derived materials
- Nerve and tissue regeneration
- PC12 cells
- Polycation
- Regulating nerve cell behavior
- Surface properties
- Surface topography
ASJC Scopus subject areas
- Biomaterials
- Polymers and Plastics
- Materials Chemistry
- Bioengineering
Cite this
Surface properties of chitosan films modified with polycations and their effects on the behavior of PC12 cells. / Zheng, Zhenhuan; Wei, Yujun; Wang, Gan; Wang, Aijun; Ao, Qiang; Gong, Yandao; Zhang, Xiufang.
In: Journal of Bioactive and Compatible Polymers, Vol. 24, No. 1, 01.2009, p. 63-82.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Surface properties of chitosan films modified with polycations and their effects on the behavior of PC12 cells
AU - Zheng, Zhenhuan
AU - Wei, Yujun
AU - Wang, Gan
AU - Wang, Aijun
AU - Ao, Qiang
AU - Gong, Yandao
AU - Zhang, Xiufang
PY - 2009/1
Y1 - 2009/1
N2 - A series of composite films were prepared by blending chitosan with three polycations, poly(L-lysine), polyethyleneimine, and poly-L-ornithine, in specific blend proportions. The surface properties of the composite films, including surface topography, chemistry, and wettability, were examined by atomic force microscopy, X-ray photoelectron spectroscopy, and contact angle assay, respectively. For all composite films, blending with different polycations produced different nanoscaled surface topographical features (particles, granules, fibers, and islands) in addition to inducing changes in surface chemistry and wettability. PC12 cells were cultured on these composite films to evaluate the effects of surface properties on cell behavior. The PC12 cell behavior was holistically affected by surface topography, chemistry, and wettability; the cells also displayed responses to surface topography. On the surfaces with fiber topographic features, the PC12 cells exhibited significantly higher levels of adhesion, proliferation, and differentiation when compared to particle, granule, or island dominant surfaces. It appears that the surface topography of chitosan and chitosan-derived materials may play an important role in regulating nerve cell behavior and that topographic modification can be utilized for the applications of chitosan and chitosan-derived materials in nerve or other tissue regeneration.
AB - A series of composite films were prepared by blending chitosan with three polycations, poly(L-lysine), polyethyleneimine, and poly-L-ornithine, in specific blend proportions. The surface properties of the composite films, including surface topography, chemistry, and wettability, were examined by atomic force microscopy, X-ray photoelectron spectroscopy, and contact angle assay, respectively. For all composite films, blending with different polycations produced different nanoscaled surface topographical features (particles, granules, fibers, and islands) in addition to inducing changes in surface chemistry and wettability. PC12 cells were cultured on these composite films to evaluate the effects of surface properties on cell behavior. The PC12 cell behavior was holistically affected by surface topography, chemistry, and wettability; the cells also displayed responses to surface topography. On the surfaces with fiber topographic features, the PC12 cells exhibited significantly higher levels of adhesion, proliferation, and differentiation when compared to particle, granule, or island dominant surfaces. It appears that the surface topography of chitosan and chitosan-derived materials may play an important role in regulating nerve cell behavior and that topographic modification can be utilized for the applications of chitosan and chitosan-derived materials in nerve or other tissue regeneration.
KW - AFM
KW - Cell behavior
KW - Chitosan
KW - Chitosan blends
KW - Chitosan-derived materials
KW - Nerve and tissue regeneration
KW - PC12 cells
KW - Polycation
KW - Regulating nerve cell behavior
KW - Surface properties
KW - Surface topography
UR - http://www.scopus.com/inward/record.url?scp=58149375714&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149375714&partnerID=8YFLogxK
U2 - 10.1177/0883911508099653
DO - 10.1177/0883911508099653
M3 - Article
AN - SCOPUS:58149375714
VL - 24
SP - 63
EP - 82
JO - Journal of Bioactive and Compatible Polymers
JF - Journal of Bioactive and Compatible Polymers
SN - 0883-9115
IS - 1
ER -