Surface electrical capacitance as an index of epidermal barrier properties of composite skin substitutes and skin autografts

Michael J. Goretsky, Andrew P. Supp, David G Greenhalgh, Glenn D. Warden, Steven T. Boyce

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Restoration of the epidermal barrier is a requirement for burn wound closure. A rapid, reliable, and noninvasive measure of the rate of restoration of the epidermal barrier is not readily available. To monitor the reformation of the epidermal barrier, we measured surface electrical capacitance on cultured skin substitutes (human keratinocytes and fibroblasts attached to collagen‐glycosaminoglycan substrates) and split‐thickness skin autografts grafted to patients. Data were collected from four patients with burns and one pediatric patient with a congenital hairy nevus comprising > 60% total body surface area. Capacitance measurements were performed at days 7, 10, 12, 14, and 28 by direct contact of the capacitance probe for 10 seconds to the cultured skin substitutes or split‐thickness autograft. On postoperative days 7, 10, 12, 14, 21, and 28, the surface electrical capacitance of cultured skin substitutes after 10 seconds of sampling was 2468 ± 268, 1443 ± 439, 129 ± 43, 200 ± 44, 88 ± 20, and 74 ± 19 picofarads (mean ± standard error of the mean), respectively. Surface electrical capacitance for split‐thickness autograft on the same days was 1699 ± 371, 1914 ± 433, 125 ± 16, 175 ± 63, 110 ± 26, 271 ± 77 picofarads, respectively. Surface electrical capacitance in all of the grafts decreased with time. Cultured skin substitutes had approximately the same 10‐second capacitance values as split‐thickness autograft during 3 weeks of healing and approached values for uninjured skin (32 ± 5 picofarads) by 12 days. Measurement of surface electrical capacitance is a direct, inexpensive, and convenient index for noninvasive monitoring of epidermal barrier formation.

Original languageEnglish (US)
Pages (from-to)419-425
Number of pages7
JournalWound Repair and Regeneration
Volume3
Issue number4
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Electric Capacitance
Artificial Skin
Autografts
Skin
Nevus
Body Surface Area
Burns
Keratinocytes
Fibroblasts
Pediatrics
Transplants
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Surface electrical capacitance as an index of epidermal barrier properties of composite skin substitutes and skin autografts. / Goretsky, Michael J.; Supp, Andrew P.; Greenhalgh, David G; Warden, Glenn D.; Boyce, Steven T.

In: Wound Repair and Regeneration, Vol. 3, No. 4, 1995, p. 419-425.

Research output: Contribution to journalArticle

Goretsky, Michael J. ; Supp, Andrew P. ; Greenhalgh, David G ; Warden, Glenn D. ; Boyce, Steven T. / Surface electrical capacitance as an index of epidermal barrier properties of composite skin substitutes and skin autografts. In: Wound Repair and Regeneration. 1995 ; Vol. 3, No. 4. pp. 419-425.
@article{6a54d149b38c4000939f26ce31027b4e,
title = "Surface electrical capacitance as an index of epidermal barrier properties of composite skin substitutes and skin autografts",
abstract = "Restoration of the epidermal barrier is a requirement for burn wound closure. A rapid, reliable, and noninvasive measure of the rate of restoration of the epidermal barrier is not readily available. To monitor the reformation of the epidermal barrier, we measured surface electrical capacitance on cultured skin substitutes (human keratinocytes and fibroblasts attached to collagen‐glycosaminoglycan substrates) and split‐thickness skin autografts grafted to patients. Data were collected from four patients with burns and one pediatric patient with a congenital hairy nevus comprising > 60{\%} total body surface area. Capacitance measurements were performed at days 7, 10, 12, 14, and 28 by direct contact of the capacitance probe for 10 seconds to the cultured skin substitutes or split‐thickness autograft. On postoperative days 7, 10, 12, 14, 21, and 28, the surface electrical capacitance of cultured skin substitutes after 10 seconds of sampling was 2468 ± 268, 1443 ± 439, 129 ± 43, 200 ± 44, 88 ± 20, and 74 ± 19 picofarads (mean ± standard error of the mean), respectively. Surface electrical capacitance for split‐thickness autograft on the same days was 1699 ± 371, 1914 ± 433, 125 ± 16, 175 ± 63, 110 ± 26, 271 ± 77 picofarads, respectively. Surface electrical capacitance in all of the grafts decreased with time. Cultured skin substitutes had approximately the same 10‐second capacitance values as split‐thickness autograft during 3 weeks of healing and approached values for uninjured skin (32 ± 5 picofarads) by 12 days. Measurement of surface electrical capacitance is a direct, inexpensive, and convenient index for noninvasive monitoring of epidermal barrier formation.",
author = "Goretsky, {Michael J.} and Supp, {Andrew P.} and Greenhalgh, {David G} and Warden, {Glenn D.} and Boyce, {Steven T.}",
year = "1995",
doi = "10.1046/j.1524-475X.1995.30406.x",
language = "English (US)",
volume = "3",
pages = "419--425",
journal = "Wound Repair and Regeneration",
issn = "1067-1927",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Surface electrical capacitance as an index of epidermal barrier properties of composite skin substitutes and skin autografts

AU - Goretsky, Michael J.

AU - Supp, Andrew P.

AU - Greenhalgh, David G

AU - Warden, Glenn D.

AU - Boyce, Steven T.

PY - 1995

Y1 - 1995

N2 - Restoration of the epidermal barrier is a requirement for burn wound closure. A rapid, reliable, and noninvasive measure of the rate of restoration of the epidermal barrier is not readily available. To monitor the reformation of the epidermal barrier, we measured surface electrical capacitance on cultured skin substitutes (human keratinocytes and fibroblasts attached to collagen‐glycosaminoglycan substrates) and split‐thickness skin autografts grafted to patients. Data were collected from four patients with burns and one pediatric patient with a congenital hairy nevus comprising > 60% total body surface area. Capacitance measurements were performed at days 7, 10, 12, 14, and 28 by direct contact of the capacitance probe for 10 seconds to the cultured skin substitutes or split‐thickness autograft. On postoperative days 7, 10, 12, 14, 21, and 28, the surface electrical capacitance of cultured skin substitutes after 10 seconds of sampling was 2468 ± 268, 1443 ± 439, 129 ± 43, 200 ± 44, 88 ± 20, and 74 ± 19 picofarads (mean ± standard error of the mean), respectively. Surface electrical capacitance for split‐thickness autograft on the same days was 1699 ± 371, 1914 ± 433, 125 ± 16, 175 ± 63, 110 ± 26, 271 ± 77 picofarads, respectively. Surface electrical capacitance in all of the grafts decreased with time. Cultured skin substitutes had approximately the same 10‐second capacitance values as split‐thickness autograft during 3 weeks of healing and approached values for uninjured skin (32 ± 5 picofarads) by 12 days. Measurement of surface electrical capacitance is a direct, inexpensive, and convenient index for noninvasive monitoring of epidermal barrier formation.

AB - Restoration of the epidermal barrier is a requirement for burn wound closure. A rapid, reliable, and noninvasive measure of the rate of restoration of the epidermal barrier is not readily available. To monitor the reformation of the epidermal barrier, we measured surface electrical capacitance on cultured skin substitutes (human keratinocytes and fibroblasts attached to collagen‐glycosaminoglycan substrates) and split‐thickness skin autografts grafted to patients. Data were collected from four patients with burns and one pediatric patient with a congenital hairy nevus comprising > 60% total body surface area. Capacitance measurements were performed at days 7, 10, 12, 14, and 28 by direct contact of the capacitance probe for 10 seconds to the cultured skin substitutes or split‐thickness autograft. On postoperative days 7, 10, 12, 14, 21, and 28, the surface electrical capacitance of cultured skin substitutes after 10 seconds of sampling was 2468 ± 268, 1443 ± 439, 129 ± 43, 200 ± 44, 88 ± 20, and 74 ± 19 picofarads (mean ± standard error of the mean), respectively. Surface electrical capacitance for split‐thickness autograft on the same days was 1699 ± 371, 1914 ± 433, 125 ± 16, 175 ± 63, 110 ± 26, 271 ± 77 picofarads, respectively. Surface electrical capacitance in all of the grafts decreased with time. Cultured skin substitutes had approximately the same 10‐second capacitance values as split‐thickness autograft during 3 weeks of healing and approached values for uninjured skin (32 ± 5 picofarads) by 12 days. Measurement of surface electrical capacitance is a direct, inexpensive, and convenient index for noninvasive monitoring of epidermal barrier formation.

UR - http://www.scopus.com/inward/record.url?scp=84989059332&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84989059332&partnerID=8YFLogxK

U2 - 10.1046/j.1524-475X.1995.30406.x

DO - 10.1046/j.1524-475X.1995.30406.x

M3 - Article

AN - SCOPUS:84989059332

VL - 3

SP - 419

EP - 425

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

SN - 1067-1927

IS - 4

ER -