Suppressors of frameshift mutations in Salmonella typhimurium

Donald L. Riddle, John R. Roth

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Suppressors specific for frameshift mutations have been studied. Of 21 frameshift mutations of the histidine operon selected for study, 13 gave rise to ICR † Abbreviations used: ICR-191, 2-chloro-6-methoxy-9-(3-(2-chloroethyl) aminopropylamino) acridine dihydrochloride; DES, diethylsulfate; 2-AP, 2-aminopurine nitrate; NG, N-methyl-N′-nitro-N-nitrosoguanidine; INT, 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride.-induced external suppressors. Five of these mutants also gave rise to nitrosoguanidine-induced suppressors. Two frameshift mutations in the tryptophan operon were found which could be suppressed externally along with certain his frameshift mutations. Most of the externally suppressible mutations ( 12 15) are those tentatively classified as (+1) frameshifts. Forty-eight independent suppressor mutations were placed in 9 groups on the basis of their ability to suppress 13 his frameshift mutations. The 13 suppressible mutations are apparently of two general types. No cross-suppression of mutations of these two types has been observed. The efficiency of suppression was estimated for at least one suppressor from each of 8 groups. The efficiency of suppression ranges from 1% to about 15%.

Original languageEnglish (US)
JournalJournal of Molecular Biology
Volume54
Issue number1
DOIs
StatePublished - Nov 28 1970

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Genetic Suppression
Frameshift Mutation
Salmonella typhimurium
Operon
Mutation
2-Aminopurine
Nitrosoguanidines
Methylnitronitrosoguanidine
Histidine
Tryptophan
Nitrates
Chlorides

ASJC Scopus subject areas

  • Virology

Cite this

Suppressors of frameshift mutations in Salmonella typhimurium. / Riddle, Donald L.; Roth, John R.

In: Journal of Molecular Biology, Vol. 54, No. 1, 28.11.1970.

Research output: Contribution to journalArticle

Riddle, Donald L. ; Roth, John R. / Suppressors of frameshift mutations in Salmonella typhimurium. In: Journal of Molecular Biology. 1970 ; Vol. 54, No. 1.
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