Abstract
Suppressors specific for frameshift mutations have been studied. Of 21 frameshift mutations of the histidine operon selected for study, 13 gave rise to ICR† † Abbreviations used: ICR-191, 2-chloro-6-methoxy-9-(3-(2-chloroethyl) aminopropylamino) acridine dihydrochloride; DES, diethylsulfate; 2-AP, 2-aminopurine nitrate; NG, N-methyl-N′-nitro-N-nitrosoguanidine; INT, 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride.-induced external suppressors. Five of these mutants also gave rise to nitrosoguanidine-induced suppressors. Two frameshift mutations in the tryptophan operon were found which could be suppressed externally along with certain his frameshift mutations. Most of the externally suppressible mutations ( 12 15) are those tentatively classified as (+1) frameshifts. Forty-eight independent suppressor mutations were placed in 9 groups on the basis of their ability to suppress 13 his frameshift mutations. The 13 suppressible mutations are apparently of two general types. No cross-suppression of mutations of these two types has been observed. The efficiency of suppression was estimated for at least one suppressor from each of 8 groups. The efficiency of suppression ranges from 1% to about 15%.
| Original language | English (US) |
|---|---|
| Journal | Journal of Molecular Biology |
| Volume | 54 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 28 1970 |
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ASJC Scopus subject areas
- Virology
Cite this
Suppressors of frameshift mutations in Salmonella typhimurium. / Riddle, Donald L.; Roth, John R.
In: Journal of Molecular Biology, Vol. 54, No. 1, 28.11.1970.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Suppressors of frameshift mutations in Salmonella typhimurium
AU - Riddle, Donald L.
AU - Roth, John R.
PY - 1970/11/28
Y1 - 1970/11/28
N2 - Suppressors specific for frameshift mutations have been studied. Of 21 frameshift mutations of the histidine operon selected for study, 13 gave rise to ICR† † Abbreviations used: ICR-191, 2-chloro-6-methoxy-9-(3-(2-chloroethyl) aminopropylamino) acridine dihydrochloride; DES, diethylsulfate; 2-AP, 2-aminopurine nitrate; NG, N-methyl-N′-nitro-N-nitrosoguanidine; INT, 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride.-induced external suppressors. Five of these mutants also gave rise to nitrosoguanidine-induced suppressors. Two frameshift mutations in the tryptophan operon were found which could be suppressed externally along with certain his frameshift mutations. Most of the externally suppressible mutations ( 12 15) are those tentatively classified as (+1) frameshifts. Forty-eight independent suppressor mutations were placed in 9 groups on the basis of their ability to suppress 13 his frameshift mutations. The 13 suppressible mutations are apparently of two general types. No cross-suppression of mutations of these two types has been observed. The efficiency of suppression was estimated for at least one suppressor from each of 8 groups. The efficiency of suppression ranges from 1% to about 15%.
AB - Suppressors specific for frameshift mutations have been studied. Of 21 frameshift mutations of the histidine operon selected for study, 13 gave rise to ICR† † Abbreviations used: ICR-191, 2-chloro-6-methoxy-9-(3-(2-chloroethyl) aminopropylamino) acridine dihydrochloride; DES, diethylsulfate; 2-AP, 2-aminopurine nitrate; NG, N-methyl-N′-nitro-N-nitrosoguanidine; INT, 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride.-induced external suppressors. Five of these mutants also gave rise to nitrosoguanidine-induced suppressors. Two frameshift mutations in the tryptophan operon were found which could be suppressed externally along with certain his frameshift mutations. Most of the externally suppressible mutations ( 12 15) are those tentatively classified as (+1) frameshifts. Forty-eight independent suppressor mutations were placed in 9 groups on the basis of their ability to suppress 13 his frameshift mutations. The 13 suppressible mutations are apparently of two general types. No cross-suppression of mutations of these two types has been observed. The efficiency of suppression was estimated for at least one suppressor from each of 8 groups. The efficiency of suppression ranges from 1% to about 15%.
UR - http://www.scopus.com/inward/record.url?scp=0014966535&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0014966535&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(70)90451-1
DO - 10.1016/0022-2836(70)90451-1
M3 - Article
C2 - 4321728
AN - SCOPUS:0014966535
VL - 54
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 1
ER -