Supplementation with ω3 Polyunsaturated Fatty Acids and all-rac Alpha-Tocopherol Alone and in Combination Failed to Exert an Anti-inflammatory Effect in Human Volunteers

Sonia Vega-López, Nalini Kaul, Sridevi Devaraj, Ru Ya Cai, Bruce German, Ishwarlal Jialal

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

There is growing evidence supporting the importance of inflammation in all stages of atherosclerosis. While both ω-3 polyunsaturated fatty acids (n3PUFA) and the lipid-soluble antioxidant alpha-tocopherol (AT) have been shown to independently have significant anti-inflammatory effects, there is paucity of data examining the effect of n3PUFA alone and in combination with AT on markers of inflammation and monocyte function. Therefore, we tested the effect of n3PUFA alone, all-rac (synthetic) AT alone, and the combination on markers of inflammation and monocyte function. Healthy nonsmoking volunteers were randomly assigned to 1 of 4 groups (n = 20 per group): 1.5 g/d n3PUFA, 800 IU/d AT, 1.5 g n3PUFA + 800 IU/d AT, or placebo in a parallel double-blinded study. Compared to baseline, 12 weeks of supplementation resulted in no changes in plasma lipids regardless of treatment. Plasma AT was significantly increased only in those groups that received AT (P < .0001). Similarly, groups receiving n3PUFA showed a significant increase in plasma docosahexaenoic acid (P < .0001). No significant within- or between-group differences were found for plasma levels of high-sensitivity C-reactive protein (hsCRP). Furthermore, there were no differences in monocyte proinflammatory cytokine release (interleukin [IL]-1β, tumor necrosis factor [TNF]-α and IL-6) after activation with monocyte chemotactic protein-1 (MCP-1). In conclusion, supplementation with n3PUFA and all-rac AT at these doses is not anti-inflammatory.

Original languageEnglish (US)
Pages (from-to)236-240
Number of pages5
JournalMetabolism: Clinical and Experimental
Volume53
Issue number2
DOIs
StatePublished - Feb 2004

Fingerprint

alpha-Tocopherol
Unsaturated Fatty Acids
Volunteers
Anti-Inflammatory Agents
Monocytes
Inflammation
Lipids
Docosahexaenoic Acids
Chemokine CCL2
Interleukin-1
C-Reactive Protein
Interleukin-6
Atherosclerosis
Healthy Volunteers
Tumor Necrosis Factor-alpha
Antioxidants
Placebos
Cytokines

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Supplementation with ω3 Polyunsaturated Fatty Acids and all-rac Alpha-Tocopherol Alone and in Combination Failed to Exert an Anti-inflammatory Effect in Human Volunteers. / Vega-López, Sonia; Kaul, Nalini; Devaraj, Sridevi; Cai, Ru Ya; German, Bruce; Jialal, Ishwarlal.

In: Metabolism: Clinical and Experimental, Vol. 53, No. 2, 02.2004, p. 236-240.

Research output: Contribution to journalArticle

Vega-López, Sonia ; Kaul, Nalini ; Devaraj, Sridevi ; Cai, Ru Ya ; German, Bruce ; Jialal, Ishwarlal. / Supplementation with ω3 Polyunsaturated Fatty Acids and all-rac Alpha-Tocopherol Alone and in Combination Failed to Exert an Anti-inflammatory Effect in Human Volunteers. In: Metabolism: Clinical and Experimental. 2004 ; Vol. 53, No. 2. pp. 236-240.
@article{7d907da954a3431aa0d2500c62370172,
title = "Supplementation with ω3 Polyunsaturated Fatty Acids and all-rac Alpha-Tocopherol Alone and in Combination Failed to Exert an Anti-inflammatory Effect in Human Volunteers",
abstract = "There is growing evidence supporting the importance of inflammation in all stages of atherosclerosis. While both ω-3 polyunsaturated fatty acids (n3PUFA) and the lipid-soluble antioxidant alpha-tocopherol (AT) have been shown to independently have significant anti-inflammatory effects, there is paucity of data examining the effect of n3PUFA alone and in combination with AT on markers of inflammation and monocyte function. Therefore, we tested the effect of n3PUFA alone, all-rac (synthetic) AT alone, and the combination on markers of inflammation and monocyte function. Healthy nonsmoking volunteers were randomly assigned to 1 of 4 groups (n = 20 per group): 1.5 g/d n3PUFA, 800 IU/d AT, 1.5 g n3PUFA + 800 IU/d AT, or placebo in a parallel double-blinded study. Compared to baseline, 12 weeks of supplementation resulted in no changes in plasma lipids regardless of treatment. Plasma AT was significantly increased only in those groups that received AT (P < .0001). Similarly, groups receiving n3PUFA showed a significant increase in plasma docosahexaenoic acid (P < .0001). No significant within- or between-group differences were found for plasma levels of high-sensitivity C-reactive protein (hsCRP). Furthermore, there were no differences in monocyte proinflammatory cytokine release (interleukin [IL]-1β, tumor necrosis factor [TNF]-α and IL-6) after activation with monocyte chemotactic protein-1 (MCP-1). In conclusion, supplementation with n3PUFA and all-rac AT at these doses is not anti-inflammatory.",
author = "Sonia Vega-L{\'o}pez and Nalini Kaul and Sridevi Devaraj and Cai, {Ru Ya} and Bruce German and Ishwarlal Jialal",
year = "2004",
month = "2",
doi = "10.1016/j.metabol.2003.09.012",
language = "English (US)",
volume = "53",
pages = "236--240",
journal = "Metabolism: Clinical and Experimental",
issn = "0026-0495",
publisher = "W.B. Saunders Ltd",
number = "2",

}

TY - JOUR

T1 - Supplementation with ω3 Polyunsaturated Fatty Acids and all-rac Alpha-Tocopherol Alone and in Combination Failed to Exert an Anti-inflammatory Effect in Human Volunteers

AU - Vega-López, Sonia

AU - Kaul, Nalini

AU - Devaraj, Sridevi

AU - Cai, Ru Ya

AU - German, Bruce

AU - Jialal, Ishwarlal

PY - 2004/2

Y1 - 2004/2

N2 - There is growing evidence supporting the importance of inflammation in all stages of atherosclerosis. While both ω-3 polyunsaturated fatty acids (n3PUFA) and the lipid-soluble antioxidant alpha-tocopherol (AT) have been shown to independently have significant anti-inflammatory effects, there is paucity of data examining the effect of n3PUFA alone and in combination with AT on markers of inflammation and monocyte function. Therefore, we tested the effect of n3PUFA alone, all-rac (synthetic) AT alone, and the combination on markers of inflammation and monocyte function. Healthy nonsmoking volunteers were randomly assigned to 1 of 4 groups (n = 20 per group): 1.5 g/d n3PUFA, 800 IU/d AT, 1.5 g n3PUFA + 800 IU/d AT, or placebo in a parallel double-blinded study. Compared to baseline, 12 weeks of supplementation resulted in no changes in plasma lipids regardless of treatment. Plasma AT was significantly increased only in those groups that received AT (P < .0001). Similarly, groups receiving n3PUFA showed a significant increase in plasma docosahexaenoic acid (P < .0001). No significant within- or between-group differences were found for plasma levels of high-sensitivity C-reactive protein (hsCRP). Furthermore, there were no differences in monocyte proinflammatory cytokine release (interleukin [IL]-1β, tumor necrosis factor [TNF]-α and IL-6) after activation with monocyte chemotactic protein-1 (MCP-1). In conclusion, supplementation with n3PUFA and all-rac AT at these doses is not anti-inflammatory.

AB - There is growing evidence supporting the importance of inflammation in all stages of atherosclerosis. While both ω-3 polyunsaturated fatty acids (n3PUFA) and the lipid-soluble antioxidant alpha-tocopherol (AT) have been shown to independently have significant anti-inflammatory effects, there is paucity of data examining the effect of n3PUFA alone and in combination with AT on markers of inflammation and monocyte function. Therefore, we tested the effect of n3PUFA alone, all-rac (synthetic) AT alone, and the combination on markers of inflammation and monocyte function. Healthy nonsmoking volunteers were randomly assigned to 1 of 4 groups (n = 20 per group): 1.5 g/d n3PUFA, 800 IU/d AT, 1.5 g n3PUFA + 800 IU/d AT, or placebo in a parallel double-blinded study. Compared to baseline, 12 weeks of supplementation resulted in no changes in plasma lipids regardless of treatment. Plasma AT was significantly increased only in those groups that received AT (P < .0001). Similarly, groups receiving n3PUFA showed a significant increase in plasma docosahexaenoic acid (P < .0001). No significant within- or between-group differences were found for plasma levels of high-sensitivity C-reactive protein (hsCRP). Furthermore, there were no differences in monocyte proinflammatory cytokine release (interleukin [IL]-1β, tumor necrosis factor [TNF]-α and IL-6) after activation with monocyte chemotactic protein-1 (MCP-1). In conclusion, supplementation with n3PUFA and all-rac AT at these doses is not anti-inflammatory.

UR - http://www.scopus.com/inward/record.url?scp=10744220611&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10744220611&partnerID=8YFLogxK

U2 - 10.1016/j.metabol.2003.09.012

DO - 10.1016/j.metabol.2003.09.012

M3 - Article

C2 - 14767877

AN - SCOPUS:10744220611

VL - 53

SP - 236

EP - 240

JO - Metabolism: Clinical and Experimental

JF - Metabolism: Clinical and Experimental

SN - 0026-0495

IS - 2

ER -