Supervised machine learning for classification of the electrophysiological effects of chronotropic drugs on human induced pluripotent stem cell-derived cardiomyocytes

Christopher Heylman, Rupsa Datta, Agua Sobrino, Steven George, Enrico Gratton

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Supervised machine learning can be used to predict which drugs human cardiomyocytes have been exposed to. Using electrophysiological data collected from human cardiomyocytes with known exposure to different drugs, a supervised machine learning algorithm can be trained to recognize and classify cells that have been exposed to an unknown drug. Furthermore, the learning algorithm provides information on the relative contribution of each data parameter to the overall classification. Probabilities and confidence in the accuracy of each classification may also be determined by the algorithm. In this study, the electrophysiological effects of β-adrenergic drugs, propranolol and isoproterenol, on cardiomyocytes derived from human induced pluripotent stem cells (hiPS-CM) were assessed. The electrophysiological data were collected using high temporal resolution 2-photon microscopy of voltage sensitive dyes as a reporter of membrane voltage. The results demonstrate the ability of our algorithm to accurately assess, classify, and predict hiPS-CM membrane depolarization following exposure to chronotropic drugs.

Original languageEnglish (US)
Article numbere0144572
JournalPLoS One
Volume10
Issue number12
DOIs
StatePublished - Dec 1 2015

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Induced Pluripotent Stem Cells
artificial intelligence
Stem cells
Cardiac Myocytes
Learning systems
drugs
Pharmaceutical Preparations
Learning algorithms
Hip
hips
Membranes
Aptitude
Depolarization
Electric potential
Photons
Isoproterenol
propranolol
Propranolol
Adrenergic Agents
Microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Supervised machine learning for classification of the electrophysiological effects of chronotropic drugs on human induced pluripotent stem cell-derived cardiomyocytes. / Heylman, Christopher; Datta, Rupsa; Sobrino, Agua; George, Steven; Gratton, Enrico.

In: PLoS One, Vol. 10, No. 12, e0144572, 01.12.2015.

Research output: Contribution to journalArticle

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