Superoxide formed from cigarette smoke impairs polymorphonuclear leukocyte active oxygen generation activity

Masahiko Tsuchiya, David F T Thompson, Yuichiro J. Suzuki, Carroll E Cross, Lester Packer

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Reactive free radicals contained in cigarette smoke (CS) and compromised phagocytic antimicrobial activities including those of polymorphonuclear leukocytes (PMNs) have been implicated in the pathogenesis of severe CS-related pulmonary disorders. In CS-exposed buffer solutions, O2 {dot minus} was the predominant generated reactive oxygen species, as demonstrated by lucigenin-amplified chemiluminescence and electron spin resonance (ESR) spin-trapping with 5,5-dimethyl-1-pyrroline N-oxide (DMPO). When PMNs were incubated in this buffer, phorbol 12-myristate 13-acetate (PMA)-stimulated active oxygen production and coupled O2 consumption were strongly impaired without appreciably affecting PMN viability (1-min exposure inhibited active oxygen production by 75%). Superoxide dismutase (SOD) totally protected and an iron chelator, diethylenetriaminepentaacetic acid (DETAPAC), also protected the CS-exposed PMNs, suggesting that generated O2 {dot minus} was an initiating factor in the impairment and OH · generation was a subsequent injurious factor. Pretreatment of PMNs with antioxidants such as α-tocopherol and dihydrolipoic acid (DHLA) was partially protective. The results suggest that (i) O2 {dot minus} is probably generated in the upper and lower respiratory tract lining fluid when they come in contact with CS; (ii) such generated O2 {dot minus} can primarily impair PMN capabilities to generate reactive oxygen species; and (iii) since these effects may contribute to the pathogenesis of CS-related lung diseases, prior supplementation with antioxidants such as α-tocopherol or DHLA might be successful in preventing these deleterious effects.

Original languageEnglish (US)
Pages (from-to)30-37
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume299
Issue number1
DOIs
StatePublished - Nov 15 1992

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Smoke
Tobacco Products
Superoxides
Reactive Oxygen Species
Neutrophils
Tocopherols
Buffers
Antioxidants
Spin Trapping
Pulmonary diseases
Chemiluminescence
Electron Spin Resonance Spectroscopy
Chelating Agents
Luminescence
Linings
Respiratory System
Contacts (fluid mechanics)
Lung Diseases
Superoxide Dismutase
Free Radicals

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Superoxide formed from cigarette smoke impairs polymorphonuclear leukocyte active oxygen generation activity. / Tsuchiya, Masahiko; Thompson, David F T; Suzuki, Yuichiro J.; Cross, Carroll E; Packer, Lester.

In: Archives of Biochemistry and Biophysics, Vol. 299, No. 1, 15.11.1992, p. 30-37.

Research output: Contribution to journalArticle

Tsuchiya, Masahiko ; Thompson, David F T ; Suzuki, Yuichiro J. ; Cross, Carroll E ; Packer, Lester. / Superoxide formed from cigarette smoke impairs polymorphonuclear leukocyte active oxygen generation activity. In: Archives of Biochemistry and Biophysics. 1992 ; Vol. 299, No. 1. pp. 30-37.
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