Abstract
The presence of superoxide dismutase in bovine and human milk was investigated by ultrafiltration, gel filtration, and isoelectric focusing. Conclusive evidence for the presence of this enzyme in both milks is presented. The molecular weight of the enzyme was estimated by gel filtration on Sephadex G-100 to be 30,000, which is consistent with reported values for the copper, zinc form of superoxide dismutase. In addition, enzyme activity was inhibited by cyanide, thus eliminating the possibility that the enzyme was present in the manganese form. Several isoenzymes were detected by isoelectric focusing in polyacrylamide gel, and the isoenzyme pattern in bovine milk was the same as that found for bovine plasma, suggesting that milk superoxide dismutase originates from plasma. It may be that the presence of copper, zinc superoxide dismutase in milk is important for the maintenance of its oxidative stability.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 221-227 |
| Number of pages | 7 |
| Journal | Biological Trace Element Research |
| Volume | 2 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1980 |
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Keywords
- copper, zinc superoxide dismutase, in milk
- gel filtration, of superoxide dismutase
- isoelectric focusing, of superoxide dismutase
- milk, superoxide dismutase in bovine and human
- plasma, and superoxide dismutase
- Superoxide dismutase, in bovine and human milk
- ultrafiltration, of superoxide dismutase
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Endocrinology
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry, medical
- Inorganic Chemistry
- Clinical Biochemistry
- Biochemistry
Cite this
Superoxide dismutase isoenzymes in bovine and human milk. / Keen, Carl L; Lönnerdal, Bo; Stein, Theodore N.; Hurley, Lucille S.
In: Biological Trace Element Research, Vol. 2, No. 3, 09.1980, p. 221-227.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Superoxide dismutase isoenzymes in bovine and human milk
AU - Keen, Carl L
AU - Lönnerdal, Bo
AU - Stein, Theodore N.
AU - Hurley, Lucille S.
PY - 1980/9
Y1 - 1980/9
N2 - The presence of superoxide dismutase in bovine and human milk was investigated by ultrafiltration, gel filtration, and isoelectric focusing. Conclusive evidence for the presence of this enzyme in both milks is presented. The molecular weight of the enzyme was estimated by gel filtration on Sephadex G-100 to be 30,000, which is consistent with reported values for the copper, zinc form of superoxide dismutase. In addition, enzyme activity was inhibited by cyanide, thus eliminating the possibility that the enzyme was present in the manganese form. Several isoenzymes were detected by isoelectric focusing in polyacrylamide gel, and the isoenzyme pattern in bovine milk was the same as that found for bovine plasma, suggesting that milk superoxide dismutase originates from plasma. It may be that the presence of copper, zinc superoxide dismutase in milk is important for the maintenance of its oxidative stability.
AB - The presence of superoxide dismutase in bovine and human milk was investigated by ultrafiltration, gel filtration, and isoelectric focusing. Conclusive evidence for the presence of this enzyme in both milks is presented. The molecular weight of the enzyme was estimated by gel filtration on Sephadex G-100 to be 30,000, which is consistent with reported values for the copper, zinc form of superoxide dismutase. In addition, enzyme activity was inhibited by cyanide, thus eliminating the possibility that the enzyme was present in the manganese form. Several isoenzymes were detected by isoelectric focusing in polyacrylamide gel, and the isoenzyme pattern in bovine milk was the same as that found for bovine plasma, suggesting that milk superoxide dismutase originates from plasma. It may be that the presence of copper, zinc superoxide dismutase in milk is important for the maintenance of its oxidative stability.
KW - copper, zinc superoxide dismutase, in milk
KW - gel filtration, of superoxide dismutase
KW - isoelectric focusing, of superoxide dismutase
KW - milk, superoxide dismutase in bovine and human
KW - plasma, and superoxide dismutase
KW - Superoxide dismutase, in bovine and human milk
KW - ultrafiltration, of superoxide dismutase
UR - http://www.scopus.com/inward/record.url?scp=0019162545&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019162545&partnerID=8YFLogxK
U2 - 10.1007/BF02785357
DO - 10.1007/BF02785357
M3 - Article
AN - SCOPUS:0019162545
VL - 2
SP - 221
EP - 227
JO - Biological Trace Element Research
JF - Biological Trace Element Research
SN - 0163-4984
IS - 3
ER -