Superoxide dismutase isoenzymes in bovine and human milk

Carl L Keen, Bo Lönnerdal, Theodore N. Stein, Lucille S. Hurley

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The presence of superoxide dismutase in bovine and human milk was investigated by ultrafiltration, gel filtration, and isoelectric focusing. Conclusive evidence for the presence of this enzyme in both milks is presented. The molecular weight of the enzyme was estimated by gel filtration on Sephadex G-100 to be 30,000, which is consistent with reported values for the copper, zinc form of superoxide dismutase. In addition, enzyme activity was inhibited by cyanide, thus eliminating the possibility that the enzyme was present in the manganese form. Several isoenzymes were detected by isoelectric focusing in polyacrylamide gel, and the isoenzyme pattern in bovine milk was the same as that found for bovine plasma, suggesting that milk superoxide dismutase originates from plasma. It may be that the presence of copper, zinc superoxide dismutase in milk is important for the maintenance of its oxidative stability.

Original languageEnglish (US)
Pages (from-to)221-227
Number of pages7
JournalBiological Trace Element Research
Volume2
Issue number3
DOIs
StatePublished - Sep 1980

Fingerprint

Human Milk
Isoenzymes
Superoxide Dismutase
Milk
Isoelectric Focusing
Enzymes
Gel Chromatography
Zinc
Copper
Gels
Plasmas
Ultrafiltration
Cyanides
Enzyme activity
Manganese
Molecular Weight
Molecular weight
Maintenance

Keywords

  • copper, zinc superoxide dismutase, in milk
  • gel filtration, of superoxide dismutase
  • isoelectric focusing, of superoxide dismutase
  • milk, superoxide dismutase in bovine and human
  • plasma, and superoxide dismutase
  • Superoxide dismutase, in bovine and human milk
  • ultrafiltration, of superoxide dismutase

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry, medical
  • Inorganic Chemistry
  • Clinical Biochemistry
  • Biochemistry

Cite this

Keen, C. L., Lönnerdal, B., Stein, T. N., & Hurley, L. S. (1980). Superoxide dismutase isoenzymes in bovine and human milk. Biological Trace Element Research, 2(3), 221-227. https://doi.org/10.1007/BF02785357

Superoxide dismutase isoenzymes in bovine and human milk. / Keen, Carl L; Lönnerdal, Bo; Stein, Theodore N.; Hurley, Lucille S.

In: Biological Trace Element Research, Vol. 2, No. 3, 09.1980, p. 221-227.

Research output: Contribution to journalArticle

Keen, CL, Lönnerdal, B, Stein, TN & Hurley, LS 1980, 'Superoxide dismutase isoenzymes in bovine and human milk', Biological Trace Element Research, vol. 2, no. 3, pp. 221-227. https://doi.org/10.1007/BF02785357
Keen, Carl L ; Lönnerdal, Bo ; Stein, Theodore N. ; Hurley, Lucille S. / Superoxide dismutase isoenzymes in bovine and human milk. In: Biological Trace Element Research. 1980 ; Vol. 2, No. 3. pp. 221-227.
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