Superior expression of juvenile hormone esterase and β-galactosidase from the basic protein promoter of Autographa californica nuclear polyhedrosis virus compared to the p10 protein and polyhedrin promoters

B. C. Bonning, P. W. Roelvink, J. M. Vlak, R. D. Possee, B. D. Hammock

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The expression characteristics of the p10, polyhedrin and basic protein promoters of Autographa californica nuclear polyhedrosis virus were compared using two reporter enzymes, juvenile hormone esterase (JHE) and β-galactosidase. In these systems, JHE is exported from the cell and β-galactosidase is localized to the cytosol. Expression of JHE from the basic, p10 and polyhedrin promoters was first detected in the medium at 13, 19 and 27 h post-infection respectively. The basic protein promoter yielded the highest expression of the three promoters tested for both enzymes, as determined by protein and enzyme activity assays. In addition, yields of β-galactosidase and JHE under control of the p10 promoter are higher relative to expression under control of the polyhedrin promoter. These data highlight the importance of investigation of viral promoters other than the polyhedrin promoter for high yield protein expression in vitro, and for insecticidal use of recombinant baculoviruses requiring high levels of expression. The results support revision of the current concept that very late viral promoters are always optimal for high yield recombinant protein expression.

Original languageEnglish (US)
Pages (from-to)1551-1556
Number of pages6
JournalJournal of General Virology
Volume75
Issue number7
StatePublished - 1994

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Galactosidases
Nucleopolyhedrovirus
Proteins
Baculoviridae
Enzyme Assays
Enzymes
Recombinant Proteins
Cytosol
juvenile hormone esterase
Infection

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Superior expression of juvenile hormone esterase and β-galactosidase from the basic protein promoter of Autographa californica nuclear polyhedrosis virus compared to the p10 protein and polyhedrin promoters. / Bonning, B. C.; Roelvink, P. W.; Vlak, J. M.; Possee, R. D.; Hammock, B. D.

In: Journal of General Virology, Vol. 75, No. 7, 1994, p. 1551-1556.

Research output: Contribution to journalArticle

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abstract = "The expression characteristics of the p10, polyhedrin and basic protein promoters of Autographa californica nuclear polyhedrosis virus were compared using two reporter enzymes, juvenile hormone esterase (JHE) and β-galactosidase. In these systems, JHE is exported from the cell and β-galactosidase is localized to the cytosol. Expression of JHE from the basic, p10 and polyhedrin promoters was first detected in the medium at 13, 19 and 27 h post-infection respectively. The basic protein promoter yielded the highest expression of the three promoters tested for both enzymes, as determined by protein and enzyme activity assays. In addition, yields of β-galactosidase and JHE under control of the p10 promoter are higher relative to expression under control of the polyhedrin promoter. These data highlight the importance of investigation of viral promoters other than the polyhedrin promoter for high yield protein expression in vitro, and for insecticidal use of recombinant baculoviruses requiring high levels of expression. The results support revision of the current concept that very late viral promoters are always optimal for high yield recombinant protein expression.",
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AU - Roelvink, P. W.

AU - Vlak, J. M.

AU - Possee, R. D.

AU - Hammock, B. D.

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