Superbeads: Immobilization in "sweet" chemistry

Jozef Nahalka, Ziye Liu, Xi Chen, Peng George Wang

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Enzymatic oligosaccharide synthesis using recombinant glycosyltransferases is able to overcome the difficulties associated with chemical methods. Nonetheless, sugar nucleotide regeneration cycles are necessary for the glycosylation. The multistep enzyme reaction can be efficiently carried out on superbeads that are prepared by immobilizing multienzyme mixtures on bead support through fused binding domains.

Original languageEnglish (US)
Pages (from-to)372-377
Number of pages6
JournalChemistry - A European Journal
Volume9
Issue number2
DOIs
StatePublished - Jan 20 2003
Externally publishedYes

Keywords

  • Enzyme catalysis
  • Immobilization
  • Oligosaccharides
  • Sugar nucleotide regeneration

ASJC Scopus subject areas

  • Chemistry(all)

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