Superbeads: Immobilization in "sweet" chemistry

Jozef Nahalka, Ziye Liu, Xi Chen, Peng George Wang

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Enzymatic oligosaccharide synthesis using recombinant glycosyltransferases is able to overcome the difficulties associated with chemical methods. Nonetheless, sugar nucleotide regeneration cycles are necessary for the glycosylation. The multistep enzyme reaction can be efficiently carried out on superbeads that are prepared by immobilizing multienzyme mixtures on bead support through fused binding domains.

Original languageEnglish (US)
Pages (from-to)372-377
Number of pages6
JournalChemistry - A European Journal
Volume9
Issue number2
DOIs
StatePublished - Jan 20 2003
Externally publishedYes

Fingerprint

Glycosylation
Oligosaccharides
Glycosyltransferases
Nucleotides
Sugars
Enzymes

Keywords

  • Enzyme catalysis
  • Immobilization
  • Oligosaccharides
  • Sugar nucleotide regeneration

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Superbeads : Immobilization in "sweet" chemistry. / Nahalka, Jozef; Liu, Ziye; Chen, Xi; Wang, Peng George.

In: Chemistry - A European Journal, Vol. 9, No. 2, 20.01.2003, p. 372-377.

Research output: Contribution to journalArticle

Nahalka, Jozef ; Liu, Ziye ; Chen, Xi ; Wang, Peng George. / Superbeads : Immobilization in "sweet" chemistry. In: Chemistry - A European Journal. 2003 ; Vol. 9, No. 2. pp. 372-377.
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