Super-resolution microscopy demystified

Lothar Schermelleh, Alexia Ferrand, Thomas R Huser, Christian Eggeling, Markus Sauer, Oliver Biehlmaier, Gregor P.C. Drummen

Research output: Contribution to journalReview article

123 Scopus citations

Abstract

Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.

Original languageEnglish (US)
Pages (from-to)72-84
Number of pages13
JournalNature Cell Biology
Volume21
Issue number1
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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    Schermelleh, L., Ferrand, A., Huser, T. R., Eggeling, C., Sauer, M., Biehlmaier, O., & Drummen, G. P. C. (2019). Super-resolution microscopy demystified. Nature Cell Biology, 21(1), 72-84. https://doi.org/10.1038/s41556-018-0251-8