[3H]Amino acid selection of aminoacyl-tRNA synthetase mutants of CHO cells: Evidence of homo- vs. hemizygosity at specific loci

Gerald M. Adair, Larry H. Thompson, Susan Fong

Research output: Contribution to journalArticle

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Abstract

Further application of the tritiated amino acid suicide selection procedure, designed to select conditional protein synthesis mutants, has yielded over 300 aminoacyl-tRNA synthetase mutants of Chinese hamster ovary cells, representing seven complementation groups. A number of these mutants and their revertants were used to examine the extent of X-chromosome linkage or other functional hemizygosity in CHO cells. Our results indicated that none of the seven different aaRS loci tested was X linked, and the the glnRS locus, unlike the asnRS and leuRS loci, does not appear to be functionally hemizygous in CHO cells.

Original languageEnglish (US)
Pages (from-to)329-343
Number of pages15
JournalSomatic Cell Genetics
Volume5
Issue number3
DOIs
StatePublished - May 1979
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

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