[3H]Amino acid selection of aminoacyl-tRNA synthetase mutants of CHO cells: Evidence of homo- vs. hemizygosity at specific loci

Gerald M. Adair; Larry H. Thompson; Susan Fong

doi:10.1007/BF01538846

[³H]Amino acid selection of aminoacyl-tRNA synthetase mutants of CHO cells: Evidence of homo- vs. hemizygosity at specific loci

Gerald M. Adair, Larry H. Thompson, Susan Fong

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Abstract

Further application of the tritiated amino acid suicide selection procedure, designed to select conditional protein synthesis mutants, has yielded over 300 aminoacyl-tRNA synthetase mutants of Chinese hamster ovary cells, representing seven complementation groups. A number of these mutants and their revertants were used to examine the extent of X-chromosome linkage or other functional hemizygosity in CHO cells. Our results indicated that none of the seven different aaRS loci tested was X linked, and the the glnRS locus, unlike the asnRS and leuRS loci, does not appear to be functionally hemizygous in CHO cells.

Original language	English (US)
Pages (from-to)	329-343
Number of pages	15
Journal	Somatic Cell Genetics
Volume	5
Issue number	3
DOIs	https://doi.org/10.1007/BF01538846
State	Published - May 1979
Externally published	Yes

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Genetics
Medicine(all)

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title = "[3H]Amino acid selection of aminoacyl-tRNA synthetase mutants of CHO cells: Evidence of homo- vs. hemizygosity at specific loci",

abstract = "Further application of the tritiated amino acid suicide selection procedure, designed to select conditional protein synthesis mutants, has yielded over 300 aminoacyl-tRNA synthetase mutants of Chinese hamster ovary cells, representing seven complementation groups. A number of these mutants and their revertants were used to examine the extent of X-chromosome linkage or other functional hemizygosity in CHO cells. Our results indicated that none of the seven different aaRS loci tested was X linked, and the the glnRS locus, unlike the asnRS and leuRS loci, does not appear to be functionally hemizygous in CHO cells.",

author = "Adair, {Gerald M.} and Thompson, {Larry H.} and Susan Fong",

year = "1979",

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T2 - Evidence of homo- vs. hemizygosity at specific loci

AU - Adair, Gerald M.

AU - Thompson, Larry H.

AU - Fong, Susan

PY - 1979/5

Y1 - 1979/5

N2 - Further application of the tritiated amino acid suicide selection procedure, designed to select conditional protein synthesis mutants, has yielded over 300 aminoacyl-tRNA synthetase mutants of Chinese hamster ovary cells, representing seven complementation groups. A number of these mutants and their revertants were used to examine the extent of X-chromosome linkage or other functional hemizygosity in CHO cells. Our results indicated that none of the seven different aaRS loci tested was X linked, and the the glnRS locus, unlike the asnRS and leuRS loci, does not appear to be functionally hemizygous in CHO cells.

AB - Further application of the tritiated amino acid suicide selection procedure, designed to select conditional protein synthesis mutants, has yielded over 300 aminoacyl-tRNA synthetase mutants of Chinese hamster ovary cells, representing seven complementation groups. A number of these mutants and their revertants were used to examine the extent of X-chromosome linkage or other functional hemizygosity in CHO cells. Our results indicated that none of the seven different aaRS loci tested was X linked, and the the glnRS locus, unlike the asnRS and leuRS loci, does not appear to be functionally hemizygous in CHO cells.

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[³H]Amino acid selection of aminoacyl-tRNA synthetase mutants of CHO cells: Evidence of homo- vs. hemizygosity at specific loci

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