Abstract
Further application of the tritiated amino acid suicide selection procedure, designed to select conditional protein synthesis mutants, has yielded over 300 aminoacyl-tRNA synthetase mutants of Chinese hamster ovary cells, representing seven complementation groups. A number of these mutants and their revertants were used to examine the extent of X-chromosome linkage or other functional hemizygosity in CHO cells. Our results indicated that none of the seven different aaRS loci tested was X linked, and the the glnRS locus, unlike the asnRS and leuRS loci, does not appear to be functionally hemizygous in CHO cells.
Original language | English (US) |
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Pages (from-to) | 329-343 |
Number of pages | 15 |
Journal | Somatic Cell Genetics |
Volume | 5 |
Issue number | 3 |
DOIs | |
State | Published - May 1979 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics
- Medicine(all)