Abstract
Further application of the tritiated amino acid suicide selection procedure, designed to select conditional protein synthesis mutants, has yielded over 300 aminoacyl-tRNA synthetase mutants of Chinese hamster ovary cells, representing seven complementation groups. A number of these mutants and their revertants were used to examine the extent of X-chromosome linkage or other functional hemizygosity in CHO cells. Our results indicated that none of the seven different aaRS loci tested was X linked, and the the glnRS locus, unlike the asnRS and leuRS loci, does not appear to be functionally hemizygous in CHO cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 329-343 |
| Number of pages | 15 |
| Journal | Somatic Cell Genetics |
| Volume | 5 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 1979 |
| Externally published | Yes |
ASJC Scopus subject areas
- Genetics
- Medicine(all)
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Adair, G. M., Thompson, L. H., & Fong, S. (1979). [3H]Amino acid selection of aminoacyl-tRNA synthetase mutants of CHO cells: Evidence of homo- vs. hemizygosity at specific loci. Somatic Cell Genetics, 5(3), 329-343. https://doi.org/10.1007/BF01538846