31P Surface‐Coil NMR Analysis of Metabolic Status in KHJJ Tumors

Dorothy A. Adams, Gerald L Denardo, Sally J. Denardo, Claire B. Conboy, E. Morton Bradbury

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Noninvasive monitoring of the effects of treatment on cancer tissue is fundamental to the development of rational radioimmunotherapy (RIT) schemes employing radiolabeled monoclonal antibodies or antibody fragments specific for human cancer. Recent advances in nuclear magnetic resonance spectroscopy make it an attractive candidate for sequential, topical monotoring by 31P NMR of metabolic events during RIT. Preliminary to this effort, we examined the metabolic competence of the well‐characterized, murine KHJJ tumor in situ in BALB/c mice using 31P surface‐coil NMR. The ATP/Pi ratio in tumor volumes ranging from 100 to 800 mm3 showed that tumors over this range of sizes were able to maintain high levels of ATP relative to Pi. As the tumor volume increased above 1 cm3, ATP/Pi levels indicated poor metabolic competence. This lack of metabolic competence was correlated with histological evidence of tissue necrosis and vascular disintegration. The T1 values of assigned phosphorus metabolites were established. Intracellular pH, as determined from the chemical shift of Pi, was found to vary in these tumors from 7.1 in rapidly metabolizing tissue to 6.6 in necrotic tumors.© 1985 Academic Press,Inc.

Original languageEnglish (US)
Pages (from-to)419-427
Number of pages9
JournalMagnetic Resonance in Medicine
Volume2
Issue number5
DOIs
StatePublished - 1985

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Mental Competency
Radioimmunotherapy
Neoplasms
Adenosine Triphosphate
Tumor Burden
Immunoglobulin Fragments
Phosphorus
Blood Vessels
Necrosis
Magnetic Resonance Spectroscopy
Monoclonal Antibodies
Therapeutics

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

31P Surface‐Coil NMR Analysis of Metabolic Status in KHJJ Tumors. / Adams, Dorothy A.; Denardo, Gerald L; Denardo, Sally J.; Conboy, Claire B.; Morton Bradbury, E.

In: Magnetic Resonance in Medicine, Vol. 2, No. 5, 1985, p. 419-427.

Research output: Contribution to journalArticle

Adams, Dorothy A. ; Denardo, Gerald L ; Denardo, Sally J. ; Conboy, Claire B. ; Morton Bradbury, E. / 31P Surface‐Coil NMR Analysis of Metabolic Status in KHJJ Tumors. In: Magnetic Resonance in Medicine. 1985 ; Vol. 2, No. 5. pp. 419-427.
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