31P NMR spectroscopy of 9L cell line in culture

Differential effect of high temperature on anchored cells and spheroids

N. Suzuki, Ingrid Kwee, L. B. Tamas, T. Nakada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The differential effects of high temperature on 9L rat gliosarcoma cells cultured in two distinctive forms, namely, anchored monolayer cells and multicellular spheroids, were investigated using 31P nuclear magnetic resonance spectroscopy. The critical temperatures resulting in irreversible changes in cellular energetics were found to be significantly different: 45°C for anchored cells and 43°C for multicellular spheroids, respectively. Phosphomonoester levels in multicellular spheroids were found to be consistently higher than those in anchored monolayer cells regardless of the temperature to which they were exposed. The study demonstrates that tissue derived from a single cell line may exhibit different metabolic properties depending on its growth pattern.

Original languageEnglish (US)
Pages (from-to)422-428
Number of pages7
JournalMagnetic Resonance in Medicine
Volume19
Issue number2
StatePublished - 1991

Fingerprint

Cellular Spheroids
Magnetic Resonance Spectroscopy
Cell Line
Temperature
Gliosarcoma
Cultured Cells
Growth

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

31P NMR spectroscopy of 9L cell line in culture : Differential effect of high temperature on anchored cells and spheroids. / Suzuki, N.; Kwee, Ingrid; Tamas, L. B.; Nakada, T.

In: Magnetic Resonance in Medicine, Vol. 19, No. 2, 1991, p. 422-428.

Research output: Contribution to journalArticle

@article{3a1e1b3e7d8649f0bb08d2ffed9c1463,
title = "31P NMR spectroscopy of 9L cell line in culture: Differential effect of high temperature on anchored cells and spheroids",
abstract = "The differential effects of high temperature on 9L rat gliosarcoma cells cultured in two distinctive forms, namely, anchored monolayer cells and multicellular spheroids, were investigated using 31P nuclear magnetic resonance spectroscopy. The critical temperatures resulting in irreversible changes in cellular energetics were found to be significantly different: 45°C for anchored cells and 43°C for multicellular spheroids, respectively. Phosphomonoester levels in multicellular spheroids were found to be consistently higher than those in anchored monolayer cells regardless of the temperature to which they were exposed. The study demonstrates that tissue derived from a single cell line may exhibit different metabolic properties depending on its growth pattern.",
author = "N. Suzuki and Ingrid Kwee and Tamas, {L. B.} and T. Nakada",
year = "1991",
language = "English (US)",
volume = "19",
pages = "422--428",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - 31P NMR spectroscopy of 9L cell line in culture

T2 - Differential effect of high temperature on anchored cells and spheroids

AU - Suzuki, N.

AU - Kwee, Ingrid

AU - Tamas, L. B.

AU - Nakada, T.

PY - 1991

Y1 - 1991

N2 - The differential effects of high temperature on 9L rat gliosarcoma cells cultured in two distinctive forms, namely, anchored monolayer cells and multicellular spheroids, were investigated using 31P nuclear magnetic resonance spectroscopy. The critical temperatures resulting in irreversible changes in cellular energetics were found to be significantly different: 45°C for anchored cells and 43°C for multicellular spheroids, respectively. Phosphomonoester levels in multicellular spheroids were found to be consistently higher than those in anchored monolayer cells regardless of the temperature to which they were exposed. The study demonstrates that tissue derived from a single cell line may exhibit different metabolic properties depending on its growth pattern.

AB - The differential effects of high temperature on 9L rat gliosarcoma cells cultured in two distinctive forms, namely, anchored monolayer cells and multicellular spheroids, were investigated using 31P nuclear magnetic resonance spectroscopy. The critical temperatures resulting in irreversible changes in cellular energetics were found to be significantly different: 45°C for anchored cells and 43°C for multicellular spheroids, respectively. Phosphomonoester levels in multicellular spheroids were found to be consistently higher than those in anchored monolayer cells regardless of the temperature to which they were exposed. The study demonstrates that tissue derived from a single cell line may exhibit different metabolic properties depending on its growth pattern.

UR - http://www.scopus.com/inward/record.url?scp=0025923838&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025923838&partnerID=8YFLogxK

M3 - Article

VL - 19

SP - 422

EP - 428

JO - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

SN - 0740-3194

IS - 2

ER -