1H NMR observation of redox potential in liver

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

1H NMR spectral editing techniques can select the distinct signals of lactate, pyruvate, β-hydroxybutyrate, and acetoacetate and provide a unique way to monitor the biochemical processes in vivo. These metabolite levels reflect the near-equilibrium dehydrogenase activity and therefore the cellular redox state. The quantitative comparison between the 1H NMR and biochemical assay data is in excellent agreement. Lactate/pyruvate and β-hydroxybutyrate/acetoacetate ratios, obtained from normalized 1H NMR spectra, respond directly to changes in the cytosolic and mitochondrial redox states. Because NMR is noninvasive, our results set the groundwork for implementing these techniques to observe tissue redox states in vivo.

Original languageEnglish (US)
Pages (from-to)11159-11165
Number of pages7
JournalBiochemistry
Volume31
Issue number45
StatePublished - 1992

Fingerprint

Liver
Hydroxybutyrates
Oxidation-Reduction
Nuclear magnetic resonance
Observation
Pyruvic Acid
Lactic Acid
Biochemical Phenomena
Oxidoreductases
Metabolites
Assays
Tissue
Proton Magnetic Resonance Spectroscopy
acetoacetic acid

ASJC Scopus subject areas

  • Biochemistry

Cite this

1H NMR observation of redox potential in liver. / Chung, Youngran; Jue, Thomas.

In: Biochemistry, Vol. 31, No. 45, 1992, p. 11159-11165.

Research output: Contribution to journalArticle

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