Abstract
The 1H-NMR signal of deoxy Mb provides a unique opportunity to measure tissue oxygenation in vivo. To utilize the technique for human application, however, requires a specific spectral characterization of both human Mb and erythrocyte Hb. We report that the proximal histidyl-NH signal of human deoxy Mb resonates at 80.3 ppm at 25°C and maintains a 3.9 ppm separation with the corresponding Hb A signal throughout the physioiogical temperature range. In the particular case of the human thenar muscle, the deoxy Mb signal is clearly detectable without any interference from Hb.
Original language | English (US) |
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Pages (from-to) | 33-37 |
Number of pages | 5 |
Journal | Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular |
Volume | 1161 |
Issue number | 1 |
DOIs | |
State | Published - Jan 15 1993 |
Keywords
- (Human heart)
- Hemoglobin
- Myoglobin
- NMR, H-
- Oxygen
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
- Structural Biology
- Medicine(all)