19F magnetic resonance imaging for stem/progenitor cell tracking with multiple unique perfluorocarbon nanobeacons

Kathryn C. Partlow, Junjie Chen, Jason A. Brant, Anne M. Neubauer, Todd E. Meyerrose, Michael H. Creer, Jan Nolta, Shelton D. Caruthers, Gregory M. Lanza, Samuel A. Wickline

Research output: Contribution to journalArticle

241 Citations (Scopus)

Abstract

MRI has been employed to elucidate the migratory behavior of stem/progenitor cells noninvasively in vivo with traditional proton ( 1H) imaging of iron oxide nanoparticle-labeled cells. Alternatively, we demonstrate that fluorine (19F) MRI of cells labeled with different types of liquid perfluorocarbon (PFC) nanoparticles produces unique and sensitive cell markers distinct from any tissue background signal. To define the utility for cell tracking, mononuclear cells harvested from human umbilical cord blood were grown under proendothelial conditions and labeled with nanoparticles composed of two distinct PFC cores (perfluorooctylbromide and perfluoro-15-crown-5 ether). The sensitivity for detecting and imaging labeled cells was defined on 11.7T (research) and 1.5T (clinical) scanners. Stem/progenitor cells (CD34+CD133+CD31+) readily internalized PFC nanoparticles without aid of adjunctive labeling techniques, and cells remained functional in vivo. PFC-labeled cells exhibited distinct 19F signals and were readily detected after both local and intravenous injection. PFC nanoparticles provide an unequivocal and unique signature for stem/progenitor cells, enable spatial cell localization with 19F MRI, and permit quantification and detection of multiple fluorine signatures via 19F MR spectroscopy. This method should facilitate longitudinal investigation of cellular events in vivo for multiple cell types simultaneously.

Original languageEnglish (US)
Pages (from-to)1647-1654
Number of pages8
JournalFASEB Journal
Volume21
Issue number8
DOIs
StatePublished - Jun 2007
Externally publishedYes

Fingerprint

perfluorocarbons
Cell Tracking
Fluorocarbons
Magnetic resonance
Stem cells
magnetic resonance imaging
stem cells
Stem Cells
Magnetic Resonance Imaging
Nanoparticles
Imaging techniques
Magnetic resonance imaging
nanoparticles
Fluorine
cells
fluorine
Labeling
Protons
Blood
image analysis

Keywords

  • Contrast agent
  • Endothelial progenitor cells
  • Nanoparticles

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology
  • Medicine(all)

Cite this

Partlow, K. C., Chen, J., Brant, J. A., Neubauer, A. M., Meyerrose, T. E., Creer, M. H., ... Wickline, S. A. (2007). 19F magnetic resonance imaging for stem/progenitor cell tracking with multiple unique perfluorocarbon nanobeacons. FASEB Journal, 21(8), 1647-1654. https://doi.org/10.1096/fj.06-6505com

19F magnetic resonance imaging for stem/progenitor cell tracking with multiple unique perfluorocarbon nanobeacons. / Partlow, Kathryn C.; Chen, Junjie; Brant, Jason A.; Neubauer, Anne M.; Meyerrose, Todd E.; Creer, Michael H.; Nolta, Jan; Caruthers, Shelton D.; Lanza, Gregory M.; Wickline, Samuel A.

In: FASEB Journal, Vol. 21, No. 8, 06.2007, p. 1647-1654.

Research output: Contribution to journalArticle

Partlow, KC, Chen, J, Brant, JA, Neubauer, AM, Meyerrose, TE, Creer, MH, Nolta, J, Caruthers, SD, Lanza, GM & Wickline, SA 2007, '19F magnetic resonance imaging for stem/progenitor cell tracking with multiple unique perfluorocarbon nanobeacons', FASEB Journal, vol. 21, no. 8, pp. 1647-1654. https://doi.org/10.1096/fj.06-6505com
Partlow, Kathryn C. ; Chen, Junjie ; Brant, Jason A. ; Neubauer, Anne M. ; Meyerrose, Todd E. ; Creer, Michael H. ; Nolta, Jan ; Caruthers, Shelton D. ; Lanza, Gregory M. ; Wickline, Samuel A. / 19F magnetic resonance imaging for stem/progenitor cell tracking with multiple unique perfluorocarbon nanobeacons. In: FASEB Journal. 2007 ; Vol. 21, No. 8. pp. 1647-1654.
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