[15N] leucine as a source of [15N] glutamate in organotypic cerebellar explants

Marc Yudkoff, Itzhak Nissim, Seung Kim, David E Pleasure, Klaus Hummeler, Stanton Segal

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Approximately 26.0% of the [15N] glutamate and [alpha 15N] glutamine formed in organotypic cerebellar explants was derived from [15N] leucine. Approximately 14.0% of the 15NH3 and [amide 15N] glutamine synthesized came from leucine nitrogen. Another 4.0% of the alpha nitrogen of both glutamate and glutamine was derived from [15N] valine. These results suggest that branched-chain amino acids, particularly leucine, may be important for the synthesis of glutamic acid by the brain.

Original languageEnglish (US)
Pages (from-to)174-179
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume115
Issue number1
DOIs
StatePublished - Aug 30 1983
Externally publishedYes

Fingerprint

Glutamine
Leucine
Glutamic Acid
Nitrogen
Branched Chain Amino Acids
Valine
Amides
Brain

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

[15N] leucine as a source of [15N] glutamate in organotypic cerebellar explants. / Yudkoff, Marc; Nissim, Itzhak; Kim, Seung; Pleasure, David E; Hummeler, Klaus; Segal, Stanton.

In: Biochemical and Biophysical Research Communications, Vol. 115, No. 1, 30.08.1983, p. 174-179.

Research output: Contribution to journalArticle

Yudkoff, Marc ; Nissim, Itzhak ; Kim, Seung ; Pleasure, David E ; Hummeler, Klaus ; Segal, Stanton. / [15N] leucine as a source of [15N] glutamate in organotypic cerebellar explants. In: Biochemical and Biophysical Research Communications. 1983 ; Vol. 115, No. 1. pp. 174-179.
@article{883715ddfc644c3c864c3410759a604b,
title = "[15N] leucine as a source of [15N] glutamate in organotypic cerebellar explants",
abstract = "Approximately 26.0{\%} of the [15N] glutamate and [alpha 15N] glutamine formed in organotypic cerebellar explants was derived from [15N] leucine. Approximately 14.0{\%} of the 15NH3 and [amide 15N] glutamine synthesized came from leucine nitrogen. Another 4.0{\%} of the alpha nitrogen of both glutamate and glutamine was derived from [15N] valine. These results suggest that branched-chain amino acids, particularly leucine, may be important for the synthesis of glutamic acid by the brain.",
author = "Marc Yudkoff and Itzhak Nissim and Seung Kim and Pleasure, {David E} and Klaus Hummeler and Stanton Segal",
year = "1983",
month = "8",
day = "30",
doi = "10.1016/0006-291X(83)90985-3",
language = "English (US)",
volume = "115",
pages = "174--179",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - [15N] leucine as a source of [15N] glutamate in organotypic cerebellar explants

AU - Yudkoff, Marc

AU - Nissim, Itzhak

AU - Kim, Seung

AU - Pleasure, David E

AU - Hummeler, Klaus

AU - Segal, Stanton

PY - 1983/8/30

Y1 - 1983/8/30

N2 - Approximately 26.0% of the [15N] glutamate and [alpha 15N] glutamine formed in organotypic cerebellar explants was derived from [15N] leucine. Approximately 14.0% of the 15NH3 and [amide 15N] glutamine synthesized came from leucine nitrogen. Another 4.0% of the alpha nitrogen of both glutamate and glutamine was derived from [15N] valine. These results suggest that branched-chain amino acids, particularly leucine, may be important for the synthesis of glutamic acid by the brain.

AB - Approximately 26.0% of the [15N] glutamate and [alpha 15N] glutamine formed in organotypic cerebellar explants was derived from [15N] leucine. Approximately 14.0% of the 15NH3 and [amide 15N] glutamine synthesized came from leucine nitrogen. Another 4.0% of the alpha nitrogen of both glutamate and glutamine was derived from [15N] valine. These results suggest that branched-chain amino acids, particularly leucine, may be important for the synthesis of glutamic acid by the brain.

UR - http://www.scopus.com/inward/record.url?scp=0020643308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020643308&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(83)90985-3

DO - 10.1016/0006-291X(83)90985-3

M3 - Article

VL - 115

SP - 174

EP - 179

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -