[15N] leucine as a source of [15N] glutamate in organotypic cerebellar explants

Marc Yudkoff, Itzhak Nissim, Seung Kim, David E Pleasure, Klaus Hummeler, Stanton Segal

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Approximately 26.0% of the [15N] glutamate and [alpha 15N] glutamine formed in organotypic cerebellar explants was derived from [15N] leucine. Approximately 14.0% of the 15NH3 and [amide 15N] glutamine synthesized came from leucine nitrogen. Another 4.0% of the alpha nitrogen of both glutamate and glutamine was derived from [15N] valine. These results suggest that branched-chain amino acids, particularly leucine, may be important for the synthesis of glutamic acid by the brain.

Original languageEnglish (US)
Pages (from-to)174-179
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume115
Issue number1
DOIs
StatePublished - Aug 30 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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