14C AMS quantification of biomolecular interactions using microbore and plate separations

M. R. Creek, C. E. Frantz, E. Fultz, K. Haack, K. Redwine, N. Shen, Ken W Turteltaub, J. S. Vogel

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

AMS directly counts radioisotopes without interference from molecular isobars. No chemical or physical information other than a bulk isotope ratio is available from the usual AMS instrument. Chemical or biological significance of the isotope ratio depends on the definition of the sample prior to conversion to material used in the AMS ion source. We use AMS as a detector for microbore and plate-based separation techniques in quantifying the binding of 14C-labeled compounds to specific DNA and protein fragments. We discuss our methods of using these microbore and plate separations of biomolecules while controlling contamination from 14C in laboratory equipment and give examples.

Original languageEnglish (US)
Pages (from-to)454-458
Number of pages5
JournalNuclear Inst. and Methods in Physics Research, B
Volume92
Issue number1-4
DOIs
StatePublished - Jun 3 1994
Externally publishedYes

Fingerprint

Alpha Magnetic Spectrometer
Isotopes
isotope ratios
Biomolecules
Ion sources
Radioisotopes
Contamination
DNA
laboratory equipment
interactions
Detectors
Proteins
isobars
ion sources
contamination
deoxyribonucleic acid
fragments
proteins
interference
detectors

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Instrumentation
  • Surfaces and Interfaces

Cite this

Creek, M. R., Frantz, C. E., Fultz, E., Haack, K., Redwine, K., Shen, N., ... Vogel, J. S. (1994). 14C AMS quantification of biomolecular interactions using microbore and plate separations. Nuclear Inst. and Methods in Physics Research, B, 92(1-4), 454-458. https://doi.org/10.1016/0168-583X(94)96053-4

14C AMS quantification of biomolecular interactions using microbore and plate separations. / Creek, M. R.; Frantz, C. E.; Fultz, E.; Haack, K.; Redwine, K.; Shen, N.; Turteltaub, Ken W; Vogel, J. S.

In: Nuclear Inst. and Methods in Physics Research, B, Vol. 92, No. 1-4, 03.06.1994, p. 454-458.

Research output: Contribution to journalArticle

Creek, M. R. ; Frantz, C. E. ; Fultz, E. ; Haack, K. ; Redwine, K. ; Shen, N. ; Turteltaub, Ken W ; Vogel, J. S. / 14C AMS quantification of biomolecular interactions using microbore and plate separations. In: Nuclear Inst. and Methods in Physics Research, B. 1994 ; Vol. 92, No. 1-4. pp. 454-458.
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