Abstract
AMS directly counts radioisotopes without interference from molecular isobars. No chemical or physical information other than a bulk isotope ratio is available from the usual AMS instrument. Chemical or biological significance of the isotope ratio depends on the definition of the sample prior to conversion to material used in the AMS ion source. We use AMS as a detector for microbore and plate-based separation techniques in quantifying the binding of 14C-labeled compounds to specific DNA and protein fragments. We discuss our methods of using these microbore and plate separations of biomolecules while controlling contamination from 14C in laboratory equipment and give examples.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 454-458 |
| Number of pages | 5 |
| Journal | Nuclear Inst. and Methods in Physics Research, B |
| Volume | 92 |
| Issue number | 1-4 |
| DOIs | |
| State | Published - Jun 3 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Surfaces, Coatings and Films
- Instrumentation
- Surfaces and Interfaces
Cite this
14C AMS quantification of biomolecular interactions using microbore and plate separations. / Creek, M. R.; Frantz, C. E.; Fultz, E.; Haack, K.; Redwine, K.; Shen, N.; Turteltaub, Ken W; Vogel, J. S.
In: Nuclear Inst. and Methods in Physics Research, B, Vol. 92, No. 1-4, 03.06.1994, p. 454-458.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 14C AMS quantification of biomolecular interactions using microbore and plate separations
AU - Creek, M. R.
AU - Frantz, C. E.
AU - Fultz, E.
AU - Haack, K.
AU - Redwine, K.
AU - Shen, N.
AU - Turteltaub, Ken W
AU - Vogel, J. S.
PY - 1994/6/3
Y1 - 1994/6/3
N2 - AMS directly counts radioisotopes without interference from molecular isobars. No chemical or physical information other than a bulk isotope ratio is available from the usual AMS instrument. Chemical or biological significance of the isotope ratio depends on the definition of the sample prior to conversion to material used in the AMS ion source. We use AMS as a detector for microbore and plate-based separation techniques in quantifying the binding of 14C-labeled compounds to specific DNA and protein fragments. We discuss our methods of using these microbore and plate separations of biomolecules while controlling contamination from 14C in laboratory equipment and give examples.
AB - AMS directly counts radioisotopes without interference from molecular isobars. No chemical or physical information other than a bulk isotope ratio is available from the usual AMS instrument. Chemical or biological significance of the isotope ratio depends on the definition of the sample prior to conversion to material used in the AMS ion source. We use AMS as a detector for microbore and plate-based separation techniques in quantifying the binding of 14C-labeled compounds to specific DNA and protein fragments. We discuss our methods of using these microbore and plate separations of biomolecules while controlling contamination from 14C in laboratory equipment and give examples.
UR - http://www.scopus.com/inward/record.url?scp=0004752857&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0004752857&partnerID=8YFLogxK
U2 - 10.1016/0168-583X(94)96053-4
DO - 10.1016/0168-583X(94)96053-4
M3 - Article
AN - SCOPUS:0004752857
VL - 92
SP - 454
EP - 458
JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
SN - 0168-583X
IS - 1-4
ER -