Sulforaphane suppresses oligomerization of TLR4 in a thiol-dependent manner

Hyung Sun Youn, Yoon Sun Kim, Zee Yong Park, So Young Kim, Na Young Choi, Sun Myung Joung, Jung A. Seo, Kyung Min Lim, Mi Kyoung Kwak, Daniel H. Hwang, Joo Young Lee

Research output: Contribution to journalArticle

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Abstract

TLRs are pattern recognition receptors that detect invading microorganisms and nonmicrobial endogenous molecules to trigger immune and inflammatory responses during host defense and tissue repair. TLR activity is closely linked to the risk of many inflammatory diseases and immune disorders. Therefore, TLR signaling pathways can provide efficient therapeutic targets for chronic diseases. Sulforaphane (SFN), anisothiocyanate, has been well known for its anti-inflammatory activities. In this study, we investigated the modulation of TLR activity by SFN and the underlying mechanism. SFN suppressed ligand-induced and ligand-independent TLR4 activation because it prevented IL-1R-associated kinase-1 degradation, activation of NF-κB and IFN regulatory factor 3, and cyclooxygenase-2 expression induced by LPS or overexpression of TLR4. Receptor oligomerization, which is one of the initial and critical events of TLR4 activation, was suppressed by SFN, resulting in the downregulation of NF-κB activation. SFN formed adducts with cysteine residues in the extracellular domain of TLR4 as confirmed by liquid chromatography-tandem mass spectrometry analysis and the inhibitory effects of SFN on oligomerization and NF-κB activation were reversed by thiol donors (DTT and N-acetyl-L-cysteine). These suggest that the reactivity of SFN to sulfhydryl moiety contributes to its inhibitory activities. Blockade of TLR4 signaling by SFN resulted in the reduced production of inflammatory cytokines and the decreased dermal inflammation and edema in vivo in experimental inflammatory animal models. Collectively, our results demonstrated that SFN downregulated TLR4 signaling through the suppression of oligomerization process in a thiol-dependent manner. These present a novel mechanism for beneficial effects of SFN and a novel anti-inflammatory target in TLR4 signaling.

Original languageEnglish (US)
Pages (from-to)411-419
Number of pages9
JournalJournal of Immunology
Volume184
Issue number1
DOIs
StatePublished - Jan 1 2010

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Sulfhydryl Compounds
Anti-Inflammatory Agents
Down-Regulation
Interferon Regulatory Factor-3
sulforafan
Ligands
Pattern Recognition Receptors
Immune System Diseases
Acetylcysteine
Cyclooxygenase 2
Tandem Mass Spectrometry
Liquid Chromatography
Cysteine
Edema
Chronic Disease
Phosphotransferases
Animal Models
Cytokines
Inflammation
Skin

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Youn, H. S., Kim, Y. S., Park, Z. Y., Kim, S. Y., Choi, N. Y., Joung, S. M., ... Lee, J. Y. (2010). Sulforaphane suppresses oligomerization of TLR4 in a thiol-dependent manner. Journal of Immunology, 184(1), 411-419. https://doi.org/10.4049/jimmunol.0803988

Sulforaphane suppresses oligomerization of TLR4 in a thiol-dependent manner. / Youn, Hyung Sun; Kim, Yoon Sun; Park, Zee Yong; Kim, So Young; Choi, Na Young; Joung, Sun Myung; Seo, Jung A.; Lim, Kyung Min; Kwak, Mi Kyoung; Hwang, Daniel H.; Lee, Joo Young.

In: Journal of Immunology, Vol. 184, No. 1, 01.01.2010, p. 411-419.

Research output: Contribution to journalArticle

Youn, HS, Kim, YS, Park, ZY, Kim, SY, Choi, NY, Joung, SM, Seo, JA, Lim, KM, Kwak, MK, Hwang, DH & Lee, JY 2010, 'Sulforaphane suppresses oligomerization of TLR4 in a thiol-dependent manner', Journal of Immunology, vol. 184, no. 1, pp. 411-419. https://doi.org/10.4049/jimmunol.0803988
Youn, Hyung Sun ; Kim, Yoon Sun ; Park, Zee Yong ; Kim, So Young ; Choi, Na Young ; Joung, Sun Myung ; Seo, Jung A. ; Lim, Kyung Min ; Kwak, Mi Kyoung ; Hwang, Daniel H. ; Lee, Joo Young. / Sulforaphane suppresses oligomerization of TLR4 in a thiol-dependent manner. In: Journal of Immunology. 2010 ; Vol. 184, No. 1. pp. 411-419.
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AU - Seo, Jung A.

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