Sulfobetaine derivatives of bile acids

Nondenaturing surfactants for membrane biochemistry

Leonard M Hjelmeland, Daniel W. Nebert, James C. Osborne

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

The syntheses of four new sulfobetaine derivatives of bile salts are presented, along with a general set of criteria for useful detergents in membrane biochemistry. Physical properties including the critical micelle concentration, aggregation number, partial specific volume, critical micellar temperature, uv-vis spectrum, and circular dichroism spectrum are examined for the new compounds. To examine the interaction of this class of compounds with macromolecules, one of these (CHAPS) was further studied. Circular dichroism spectra of apolipoprotein C-III2 were measured in the presence of varying concentrations of CHAPS to determine the effect of this compound on secondary structure. Gel-exclusion chromatography and sedimentation equilibrium studies of cytochrome P-450 in the presence of CHAPS were also performed to establish the ability of this detergent to disaggregate cytochrome P-450 to a monomeric/dimeric state.

Original languageEnglish (US)
Pages (from-to)72-82
Number of pages11
JournalAnalytical Biochemistry
Volume130
Issue number1
DOIs
StatePublished - Apr 1 1983
Externally publishedYes

Fingerprint

Biochemistry
Bile Acids and Salts
Surface-Active Agents
Circular Dichroism
Derivatives
Membranes
Detergents
Cytochrome P-450 Enzyme System
Gel Chromatography
Apolipoproteins C
Critical micelle concentration
Micelles
Chromatography
Macromolecules
Sedimentation
Density (specific gravity)
Agglomeration
Physical properties
Gels
Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sulfobetaine derivatives of bile acids : Nondenaturing surfactants for membrane biochemistry. / Hjelmeland, Leonard M; Nebert, Daniel W.; Osborne, James C.

In: Analytical Biochemistry, Vol. 130, No. 1, 01.04.1983, p. 72-82.

Research output: Contribution to journalArticle

@article{13fb259f50ac4ba1a7942542b31186c4,
title = "Sulfobetaine derivatives of bile acids: Nondenaturing surfactants for membrane biochemistry",
abstract = "The syntheses of four new sulfobetaine derivatives of bile salts are presented, along with a general set of criteria for useful detergents in membrane biochemistry. Physical properties including the critical micelle concentration, aggregation number, partial specific volume, critical micellar temperature, uv-vis spectrum, and circular dichroism spectrum are examined for the new compounds. To examine the interaction of this class of compounds with macromolecules, one of these (CHAPS) was further studied. Circular dichroism spectra of apolipoprotein C-III2 were measured in the presence of varying concentrations of CHAPS to determine the effect of this compound on secondary structure. Gel-exclusion chromatography and sedimentation equilibrium studies of cytochrome P-450 in the presence of CHAPS were also performed to establish the ability of this detergent to disaggregate cytochrome P-450 to a monomeric/dimeric state.",
author = "Hjelmeland, {Leonard M} and Nebert, {Daniel W.} and Osborne, {James C.}",
year = "1983",
month = "4",
day = "1",
doi = "10.1016/0003-2697(83)90651-6",
language = "English (US)",
volume = "130",
pages = "72--82",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Sulfobetaine derivatives of bile acids

T2 - Nondenaturing surfactants for membrane biochemistry

AU - Hjelmeland, Leonard M

AU - Nebert, Daniel W.

AU - Osborne, James C.

PY - 1983/4/1

Y1 - 1983/4/1

N2 - The syntheses of four new sulfobetaine derivatives of bile salts are presented, along with a general set of criteria for useful detergents in membrane biochemistry. Physical properties including the critical micelle concentration, aggregation number, partial specific volume, critical micellar temperature, uv-vis spectrum, and circular dichroism spectrum are examined for the new compounds. To examine the interaction of this class of compounds with macromolecules, one of these (CHAPS) was further studied. Circular dichroism spectra of apolipoprotein C-III2 were measured in the presence of varying concentrations of CHAPS to determine the effect of this compound on secondary structure. Gel-exclusion chromatography and sedimentation equilibrium studies of cytochrome P-450 in the presence of CHAPS were also performed to establish the ability of this detergent to disaggregate cytochrome P-450 to a monomeric/dimeric state.

AB - The syntheses of four new sulfobetaine derivatives of bile salts are presented, along with a general set of criteria for useful detergents in membrane biochemistry. Physical properties including the critical micelle concentration, aggregation number, partial specific volume, critical micellar temperature, uv-vis spectrum, and circular dichroism spectrum are examined for the new compounds. To examine the interaction of this class of compounds with macromolecules, one of these (CHAPS) was further studied. Circular dichroism spectra of apolipoprotein C-III2 were measured in the presence of varying concentrations of CHAPS to determine the effect of this compound on secondary structure. Gel-exclusion chromatography and sedimentation equilibrium studies of cytochrome P-450 in the presence of CHAPS were also performed to establish the ability of this detergent to disaggregate cytochrome P-450 to a monomeric/dimeric state.

UR - http://www.scopus.com/inward/record.url?scp=0020597722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020597722&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(83)90651-6

DO - 10.1016/0003-2697(83)90651-6

M3 - Article

VL - 130

SP - 72

EP - 82

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -