Abstract
The syntheses of four new sulfobetaine derivatives of bile salts are presented, along with a general set of criteria for useful detergents in membrane biochemistry. Physical properties including the critical micelle concentration, aggregation number, partial specific volume, critical micellar temperature, uv-vis spectrum, and circular dichroism spectrum are examined for the new compounds. To examine the interaction of this class of compounds with macromolecules, one of these (CHAPS) was further studied. Circular dichroism spectra of apolipoprotein C-III2 were measured in the presence of varying concentrations of CHAPS to determine the effect of this compound on secondary structure. Gel-exclusion chromatography and sedimentation equilibrium studies of cytochrome P-450 in the presence of CHAPS were also performed to establish the ability of this detergent to disaggregate cytochrome P-450 to a monomeric/dimeric state.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 72-82 |
| Number of pages | 11 |
| Journal | Analytical Biochemistry |
| Volume | 130 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 1 1983 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Sulfobetaine derivatives of bile acids : Nondenaturing surfactants for membrane biochemistry. / Hjelmeland, Leonard M; Nebert, Daniel W.; Osborne, James C.
In: Analytical Biochemistry, Vol. 130, No. 1, 01.04.1983, p. 72-82.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sulfobetaine derivatives of bile acids
T2 - Nondenaturing surfactants for membrane biochemistry
AU - Hjelmeland, Leonard M
AU - Nebert, Daniel W.
AU - Osborne, James C.
PY - 1983/4/1
Y1 - 1983/4/1
N2 - The syntheses of four new sulfobetaine derivatives of bile salts are presented, along with a general set of criteria for useful detergents in membrane biochemistry. Physical properties including the critical micelle concentration, aggregation number, partial specific volume, critical micellar temperature, uv-vis spectrum, and circular dichroism spectrum are examined for the new compounds. To examine the interaction of this class of compounds with macromolecules, one of these (CHAPS) was further studied. Circular dichroism spectra of apolipoprotein C-III2 were measured in the presence of varying concentrations of CHAPS to determine the effect of this compound on secondary structure. Gel-exclusion chromatography and sedimentation equilibrium studies of cytochrome P-450 in the presence of CHAPS were also performed to establish the ability of this detergent to disaggregate cytochrome P-450 to a monomeric/dimeric state.
AB - The syntheses of four new sulfobetaine derivatives of bile salts are presented, along with a general set of criteria for useful detergents in membrane biochemistry. Physical properties including the critical micelle concentration, aggregation number, partial specific volume, critical micellar temperature, uv-vis spectrum, and circular dichroism spectrum are examined for the new compounds. To examine the interaction of this class of compounds with macromolecules, one of these (CHAPS) was further studied. Circular dichroism spectra of apolipoprotein C-III2 were measured in the presence of varying concentrations of CHAPS to determine the effect of this compound on secondary structure. Gel-exclusion chromatography and sedimentation equilibrium studies of cytochrome P-450 in the presence of CHAPS were also performed to establish the ability of this detergent to disaggregate cytochrome P-450 to a monomeric/dimeric state.
UR - http://www.scopus.com/inward/record.url?scp=0020597722&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020597722&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(83)90651-6
DO - 10.1016/0003-2697(83)90651-6
M3 - Article
VL - 130
SP - 72
EP - 82
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -