Successful six-day kidney preservation using trophic factor supplemented media and simple cold storage

Jonathan F. McAnulty, Ted W. Reid, Ken R. Waller, Christopher J Murphy

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

This study examined the effect of trophic factor supplementation [TFS; bovine neutrophil peptide-1 (bactenecin), 1 mg/L; substance P, 2.5 mg/L; nerve growth factor, 20μg/L; epidermal growth factor, 10μg/L; insulin-like growth factor-1, 10μg/L] during cold storage with UW lactobionate solution. Dogs transplanted with kidneys stored for 4days in TFS-UW had significantly lower peak serum creatinine values (mean 2.9±0.2mg/dL) and returned to normal values faster (6days) than kidneys stored for 3days in unmodified UW solution (4.2±0.3 mg/dL and 14days, respectively). Kidneys stored for 5days in TFS-UW (mean peak creatinine 3.7±0.3) functioned equivalently to kidneys stored for 3days and better than kidneys stored for 4days in UW alone. Dogs with kidneys stored for 6days in TFS-UW had mean peak creatinines of 5.7±0.4mg/dL. These returned to normal creatinine values in 14days, equal to 3-day stored and significantly better than kidneys stored for 4days in UW alone (20days recovery time). This study shows trophic factor deprivation appears to be a critical mechanism of injury in organ preservation with current synthetic storage media, and marks the initial development of a synthetic biologically active preservation solution, the next generation of preservation media.

Original languageEnglish (US)
Pages (from-to)712-718
Number of pages7
JournalAmerican Journal of Transplantation
Volume2
Issue number8
DOIs
StatePublished - Sep 2002
Externally publishedYes

Keywords

  • Bactenecin
  • Cold storage
  • Dog
  • Epidermal growth factor
  • Insulin-like growth factor-1
  • Kidney
  • Nerve growth factor
  • Substance P
  • Transplantation
  • Trophic factors

ASJC Scopus subject areas

  • Immunology

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