Subunit contacts of the rifamycin binding site of RNA polymerase (B. subtilis)

Lyle S. Rice, Claude F. Meares

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The radiolabeled RNA polymerase inhibitors 3-(2-bromoacetamidoethyl)-thiorifamycin and 3-(2-acetamidoethyl)-thiorifamycin quinone have been prepared and covalently attached to the B. subtilis enzyme under mild conditions. Analysis of the subunits labeled indicates that regions of subunits sigma, beta, and beta-prime lie within about 7Å of the 3-position of rifamycin bound to the enzyme, while subunits alpha, beta, and beta-prime lie near the aromatic rings. The results of this work imply that the rifamycin binding site lies near the center of an arrangement involving at least four of the subunits of RNA polymerase. This idea is supported by the results of other studies involving cross-linking of subunits and also rifamycin binding to subunit complexes.

Original languageEnglish (US)
Pages (from-to)26-32
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume80
Issue number1
DOIs
StatePublished - Jan 13 1978

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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